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Degradation of Bisphenol A and Removal of Its Estrogenic Activity by Two Laccase Transformants of Irpex lacteus  

Kim, Yun-Jung (Division of Life Sciences, Kangwon National University)
Song, Hong-Gyu (Division of Life Sciences, Kangwon National University)
Choi, Hyoung-T. (Division of Life Sciences, Kangwon National University)
Publication Information
Korean Journal of Microbiology / v.44, no.3, 2008 , pp. 199-202 More about this Journal
Abstract
A white rot fungus Irpex lacteus produced lignin degrading enzymes, which showed degrading activity against various recalcitrant compounds. However, laccase, one of the lignin degrading enzymes, was too low to be assayed by spectrophotometry using o-tolidine as the chromogenic substrate in this fungus under various culture conditions. A laccase expression vector was constructed using a cDNA from Phlebia tremellosa with the constitutively expressed promoter of glyceraldehydes-3-phosphate dehydrogenase gene, and introduced into I. lacteus by the restriction enzyme mediated integration transformation through the protoplast-$CaCl_2$ procedure. Two transformants showed highly increased laccase activities at the early growth phase in the minimal liquid medium, and they not only degraded bisphenol A, a notorious endocrine disrupting chemical, but also removed the estrogenic activity effectively.
Keywords
bisphenol A; endocrine disrupting chemicals; estrogenic activity; genetic transformation; Irpex lacteus;
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