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Construction of spDbp5 Null Mutants Defective in mRNA Export  

Bae, Jin-Ah (Basic Science Research Institute and Department of Biology, College of Natural Sciences, Sungshin Women's University)
Cho, Hyun-Jin (Basic Science Research Institute and Department of Biology, College of Natural Sciences, Sungshin Women's University)
Yoon, Jin-Ho (Basic Science Research Institute and Department of Biology, College of Natural Sciences, Sungshin Women's University)
Publication Information
Korean Journal of Microbiology / v.44, no.1, 2008 , pp. 80-84 More about this Journal
Abstract
We constructed the null mutants of fission yeast Schizosaccharomyces pombe spDbp5 gene that is homologous to DEAD-box RNA helicase DBP5 in budding yeast Saccharomyces cerevisiae, which plays important roles in mRNA export out of nucleus. A null mutant in an $h^+/h^+$ diploid strain was constructed by replacing the spDbp5-coding region with an $ura4^+$ gene using one-step gene disruption method. Tetrad analysis showed that the spDbp5 is essential for vegetative growth. The haploid spDbp5 null mutants harboring pREP81X-spDbp5 plasmid showed extensive $poly(A)^+$ RNA accumulation in the nucleus and decrease in the cytoplasm after repression of spDbp5 expression. These results suggest that spDbp5 is also involved in mRNA export from the nucleus.
Keywords
deletion mutant; mRNA export; Schizosaccharomyces pombe; spDbp5;
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