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Serological Analysis of the Antigenicity during Cultivation of Streptomyces Strains  

Kim, Jae-Heon (Department of Microbiology and Institute of Basic Sciences, Dankook University)
Jo, Sung-Kee (Radiation Research Center for Biotechnology, Korea Atomic Energy Research Institute)
Publication Information
Korean Journal of Microbiology / v.43, no.4, 2007 , pp. 321-324 More about this Journal
Abstract
The changes in antigenicity during cultivation of streptomycetes were determined by immunodiffusion assay and indirect ELISA. New precipitin lines in immunodiffusion assay began to appear in the growth period of the soluble pigment production and became thickened thereafter. The increase in the antigenicity was also confirmed by ELISA. The antigenic development was relatively weak for S. lavendulae and S. viridochromogenes while that was strong for S. lavendulae and S. viridochromogenes. The results indicated that Streptomyces strains, even though not proved for some strains, changed the compositions of cell envelope during submerged growth and this could be estimated quantitatively by serological method.
Keywords
antigenicity; envelope antigen; growth phase; Streptomyces;
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