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Biodegradation of Phenanthrene by Transformant Trametes versicolor MrP1  

Choi, Yun-Seong (Division of Life Sciences, Research Institute of Life Sciences, Kangwon National University)
Choi, Hyoung-Tae (Division of Life Sciences, Research Institute of Life Sciences, Kangwon National University)
Song, Hong-Gyu (Division of Life Sciences, Research Institute of Life Sciences, Kangwon National University)
Publication Information
Korean Journal of Microbiology / v.43, no.4, 2007 , pp. 273-278 More about this Journal
Abstract
As a model compound of PAHs (polycyclic aromatic hydrocarbons) phenanthrene has been regarded as a toxic material, mutagen and carcinogen in various animals. Biodegradation conditions of phenanthrene such as pH, temperature, shaking speed, stabilizer and cofactor of degrading enzymes were investigated with Trametes versicolor and its transformant T. versicolor MrP1 in YMG medium, minimal medium and soil microcosm. T. versicolor MrP1 can overexpress mrp gene encoding Mn-repressed peroxidase that is involved in fungal degradation. Biodegradations of phenanthrene by T. versicolor and T. versicolor MrP1 were optimally performed in conditions of weak-acid (pH 6.0), $30^{\circ}C$, shaken culture and medium containing 5 mM veratryl alcohol or tryptophan. In these optimal conditions, biodegradation of phenanthrene by T. versicolor MrP1 is 31% higher than that of wild type strain in a minimal medium for 20 days. Biodegradation of phenanthrene by T. versicolor MrP1 was also higher than that of wild type in soil microcosm. T. versicolor MrP1 can be a excellent candidate for the bioremediation of PAHs contaminated environments.
Keywords
biodegradation; phenanthrene; Trametes versicolor; transformant;
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1 Alexander, M. 1999. Biodegradation and Bioremediation, 2nd (ed.), pp. 282-287, 299-301. Academic Press, New York, USA
2 Han, M.-J., H.T. Choi, and H.-G. Song. 2004. Degradation of phenanthrene by Trametes vericolor and its laccase. J. Microbiol. 42, 94-98
3 Pazarlioglu, N.K., M. Sariisik, and A. Telefoncu. 2004. Laccase: production by Trametes versicolor and application to denim washing. Process Biochem. 40, 1673-1678   DOI   ScienceOn
4 Reddy, C.A. 1995. The potential for white-rot fungi in the treatment of pollutants. Curr. Opin. Biotechnol. 6, 320-328   DOI   ScienceOn
5 Rodgers, J.D. and N.J. Bunce. 2001. Treatment methods for the remediation of nitroaromatic explosives. Water Res. 35, 2101-2111   DOI   ScienceOn
6 Won, W.D., L.H. Disalvo, and J. Ng. 1976. Toxicity and mutagenicity of 2,4,6-trinitrotoluene and its microbial metabolites. Appl. Environ. Microbiol. 31, 576-580   PUBMED
7 Bezalel, L., Y. Hadar, P.P. Fu, J.P. Freeman, and C.E. Cerniglia. 1996. Metabolism of phenanthrene by the white rot fungus Pleurotus ostreatus. Appl. Environ. Microbiol. 62, 2547-2553   PUBMED
8 Yeo, S., N. Park, H.-G. Song, and H.T. Choi. 2007. Generation of a transformant showing higher manganese peroxidase (Mnp) activity by overexpression of mnp gene in Trametes versicolor. J. Microbiol. 45, 213-218   과학기술학회마을
9 Chang, B.V., L.C. Shiung, and S.Y. Yuan. 2002. Anaerobic biodegradation of polycyclic aromatic hydrocarbon in soil. Chemosphere 48, 717-724   DOI   ScienceOn
10 Kayali-Sayadi, M.N., S. Rubio-Barroso, C.A. Diaz-Diaz, and L.M. Polo-Diez. 2000. Rapid determination of PAHs in soil samples by HPLC with fluorimetric detection following sonication extraction. Fresenius J. Anal. Chem. 368, 697-701   DOI
11 Wilson, S.C. and K.C. Jones. 1993. Bioremediation of soils contaminated with polynuclear aromatic hydrocarbons (PAHs). Environ. Poll. 88, 229-249
12 Cerniglia, C.E. 1992. Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation 3, 351-368   DOI
13 Tien, K. and T. Kirk. 1988. Lignin peroxidase of Phanerochaete chrysosporium. Methods Enzymol. 161, 813-817
14 Collins, P.J., J.A. Field, P. Teunissen, and A.D. Dobson. 1997. Stabilization of lignin peroxidases in white-rot fungi by tryptophan. Appl. Environ. Microbiol. 63, 2543-2548   PUBMED
15 Baborova, P., M. Moder, P. Baldrian, K. Cajthamlova, and T. Cajthaml. 2006. Purification of a new manganese peroxidase of the white-rot fungus Irpex lacteus, and degradation of polycyclic aromatic hydrocarbons by the enzyme. Res. Microbiol. 157, 248-253   DOI   ScienceOn
16 Kim, H.-Y. and H.-G. Song. 2000. Comparison of 2,4,6-trinitrotoluene degradation by seven strains of white rot fungi. Curr. Microbiol. 41, 317-320   DOI
17 Faison, B.D., T.K. Kirk, and R.L. Farrell. 1986. Role of veratryl alcohol in regulating ligninase activity in Phanerochaete chrysosporium. Appl. Environ. Microbiol. 52, 251-254   PUBMED
18 Levin, L., A. Viale, and A. Forchiassin. 2003. Degradation of organic pollutants by the white rot basidiomycete Trametes trogii. Int. Biodet. Biodeg. 52, 1-5   DOI   ScienceOn
19 Kim, Y., S. Yeo, J. Kum, H.-G. Song, and H.T. Choi. 2005. Cloning of a manganese peroxidase cDNA gene repressed by manganese in Trametes versicolor. J. Microbiol. 43, 569-571   과학기술학회마을
20 Gibson, D.T., J.R. Koch, and R.E. Kallio. 1968. Oxidative degradation of aromatic hydrocarbons by microorganisms. I. Enzymatic formation of catechol from benzene. Biochemistry 7, 2653-2661   DOI   ScienceOn