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Characteristics of Vibrio vulnificus Isolated in Incheon  

Oh, Bo-Young (Incheon Institute of Health and Environment)
Kim, Jung-Hee (Incheon Institute of Health and Environment)
Gong, Young-Woo (Incheon Institute of Health and Environment)
JeGal, Seung (Incheon Institute of Health and Environment)
Kim, Hye-Yeung (Incheon Institute of Health and Environment)
Lee, Mi-Yeon (Incheon Institute of Health and Environment)
Hwang, Kyoung-Wha (Incheon Institute of Health and Environment)
Koh, Yeon-Ja (Incheon Institute of Health and Environment)
Lee, Jae-Mann (Incheon Institute of Health and Environment)
Go, Jong-Myoung (Incheon Institute of Health and Environment)
Kim, Yong-Hee (Incheon Institute of Health and Environment)
Publication Information
Korean Journal of Microbiology / v.43, no.4, 2007 , pp. 256-263 More about this Journal
Abstract
We performed the biochemical characteristics, molecular epidemiologocal analysis, and drug susceptibility test on V. vulnificus isolated from environmental sources in Incheon. For this study, 233 strains were isolated from seawater, sediment, shellfish. V. vulnificus isolates were divided into 15 biochemical groups, which were positive for ONPG and Amygdalin test. Among the 209 strains, 206 (98.6%) strains and 110 (52.6%) strains revealed positive for vvhA and viuB gene, and the viuB gene detection rates of V. vulnificus from seawater, shellfish and sediment were 48%, 48.5% and 61.6%, respectively. From disc diffusion test on 175 isolates, most of strains were sensitive to Imipenem (100.0%), Sulfamethoxazole/trimethoprim (98.9%), Tetracycline, Ciprofloxacin (98.3%), Ampicillin/sulbactam (97.1%), Ohloramphenicol (96.6%), Cefepime (94.9%) and Ceftriaxone (94.8%), multi-drug resistance rates was 31.5% of seawater, 34.4% of sediment and 29.2% of shellfish. PFGE was performed on 233 V. vulnificus isolates with the objective of investigating the extent of genetic diversity of these isolates in our region. We could find that at least 126 different PFGE patterns were generated according by 90% of similarity and 13 clusters by 58% of similarity. The major cluster was type I (44.6%) during the most of the year, and type J was frequent pattern in June and October. There were 9 distinct PFGE types in July, 8 types in August, 7 types in June, 6 types in September, 5 types in October 3 types in May and 1 type in March.
Keywords
antibiotic resistance; PFGE; viuB; vvhA; V. vulnificus;
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