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Isolation and Charcterization of Micrococcus sp. HJ-19 Secreting Extracellular Protease  

Cha, In-Tae (Department of Microbiology, Chungbuk National University)
Oh, Yong-Sik (Department of Microbiology, Chungbuk National University)
Roh, Dong-Hyun (Department of Microbiology, Chungbuk National University)
Publication Information
Korean Journal of Microbiology / v.43, no.3, 2007 , pp. 222-226 More about this Journal
Abstract
Proteases are degradative enzymes which hydrolyze a peptide bond between amino acids and they are abundantly applied to commercial field. In order to screen new source of pretense, bacteria secreting extracellular pretense were isolated by enrichment culture from deep sea water samples of East Sea, Korea. A bacterium, named as HJ19, showed the best growth and the largest clear zone in plates supplemented skim milk at $30^{\circ}C$. The partial DNA sequence analysis of the 16S rRNA gene, phenotypic tests and morphology identified that this strain was In genus Micrococcus. The strain HJ19 could not grow at $10^{\circ}C$ but it started growth and showed pretense activity at $20^{\circ}C$. The optimal growth was at $37^{\circ}C$ and the maximal protease activity at $30^{\circ}C$ was about 480unit/ml.
Keywords
extracellular pretense; growth characterization; Micrococcus sp.; 16S rRNA gene;
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