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Isolation and Identification of Rumen Fungus and Its Produced Conjugated Linoleic Acid  

Nam, In-Sik (National Livestock Research Institute, RDA)
Publication Information
Korean Journal of Microbiology / v.43, no.2, 2007 , pp. 111-115 More about this Journal
Abstract
The aim of this study was to isolate and identify men fungus which produces conjugated linoleic acid. IS-13 fungus hydrogenated conjugated linoleic acid and trans-11 vaccenic acid within 12 hr after addition of linoleic acid. The homology of IS-13 rumen fungus was compared with internal transcribed spacer 1 region (ITS1)sequences of twenty three men fungi. The length of ITS1 region of IS-13 isolate was 218 bp. IS-13 isolate has the most similar sequence (98% matched) with Orpinomyces species according to maximum-likehood and distance matrix results. The result supported that IS-13 isolate belonged to Orpinomyces genus.
Keywords
biohydrogenation; conjugated linoleic acid; isolation; Orpinomyces species; rumen fungus;
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