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Isolation and Identiffication of Acinetobacter koreensis sp. nov. from Jang-Baek Waterfall  

Lee, Ha-Yan (Department of Biological Engineering, Kyonggi University)
Yoo, Yong-Kyu (Korea Environmental Microorganism Center, Kyonggi University)
Seo, Pil-Soo (Depart-ment of Korea Biological Resourse Center, Kyonggi University)
Lee, Jung-Sook (Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology)
Lee, Keun-Chul (Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology)
Lee, Sang-Seob (Department of Biological Engineering, Kyonggi University)
Publication Information
Korean Journal of Microbiology / v.43, no.1, 2007 , pp. 66-71 More about this Journal
Abstract
Two isolates of genus Acinetobacter were obtained from Jang-Baek waterfall in North Korea. Morphological characteristics of the isolated 2 strains were Gram-negative, aerobic and rod shape bacteria. Physiological and biochemical characterization of the isolated 2 strains were some different aspect from those of type strains. 16S rDNA sequence analysis showed that the two isolates shared 99.9% sequence similarity. Strains JB10 and $JB15^{T}$ were shown to belong to the Gammaproteobacteria and showed the highest levels of sequence similarity to Acinetobacter tandoii $4N13^{T}$ (97.3%), Acinetobacter haemolyticus $ATCC17906^{T}$ (97.2%), Acinetobacter johnsonii $DSM6963^{T}$ (97.2%), Acinetobacter junii $DSM6964^{T}$ (96.7%), Acinetobacter schindleri $LUH5832^{T}$ (97.0%) and Acinetobacter ursingii $LUH3792^{T}$ (96.6%). The major cellular fatty acid in Acinetobacter type strains and isolated strains included $C_{18:1}\;{\omega}9c\;and\;C_{16:1}\;{\omega}7c/C_{15:0}\;iso\;2OH$. Eventhough it was ascertained that the isolated strains were closely related to genus Acinetobacter, physiological and biochemical characteristics and the result of the isolated strains 16S rDNA analysis indicate some different aspects from those of type strains of genus Acinetohacter It is considered that the isolated JB10 (=KEMC 52-093) and $JB15^{T}\;(=KEMC\;52-094^{T})$ strains be new species of genus Acinetobacter. We name it as Acinetobacter koreensis sp. nov.
Keywords
16S rDNA sequence; Acinetobacter koreensis; fatty acid;
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