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Analysis of Syncytium Formation Mechanism induced by Ecotropic Murine Retrovirus  

Bae, Eun-Hye (Department of Microbiology and Institute Basic Science, College of Advanced Science, Dankook University)
Park, Sung-Han (Department of Microbiology and Institute Basic Science, College of Advanced Science, Dankook University)
Jung, Yong-Tae (Department of Microbiology and Institute Basic Science, College of Advanced Science, Dankook University)
Publication Information
Korean Journal of Microbiology / v.42, no.3, 2006 , pp. 230-234 More about this Journal
Abstract
To study the mechanism of syncytium formation, novel syncytia-inducing ecotropic murine retrovirus was used. Our previous result showed that amino acid substitutions at the RBD (receptor binding domain) of envelope glycoprotein contribute to syncytium formation. In this study, we have investigated if this fusion phenomenon could occur with retroviral vectors pseudotyped with the novel syncytia-inducing ecotropic murine leukemia virus Env. We have found that these vectors were not able to mediate virus-to-cell fusion in M. dunni murine cell lines. These findings indicate that syncytia-inducing ecotropic murine leukemia virus is capable of generating syncytia during its replication. There was also no correlation between the level of ecotropic murine leukemia virus receptor (mCAT-1) and the fusogenic effect.
Keywords
ecotropic mouse retrovirus; envelope; mCAT receptor; M. dunni; syncytium;
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1 Eckert, D.M. and P.S. Kim. 2001. Mechanisms of viral membrane fusion and its inhibition. Annu. Rev. Biochem. 70, 777-810   DOI   ScienceOn
2 Kim, J.W., E.I. Closs, L.M. Albritton, and J.M. Cunningham. 1991. Transport of cationic amino acids by the mouse ecotropic retrovirus receptor. Nature 352, 725-728   DOI   ScienceOn
3 White, J., M. Kielian, and A. Helenius. 1983. Membrane fusion proteins of enveloped animal viruses. Q. Rev. Biophys. 16, 151-195   DOI
4 Masuda, M., N. Kakushima, S.G. Wilt, S.K. Ruscetti, P.M. Hoffman, A. Iwamoto, and M. Masuda. 1999. Analysis of receptor usage by ecotropic murine retroviruses, using green fluorescent protein-tagged cationic amino acid transporters. J. Virol. 73, 8623- 8629
5 Anderson, K.B. and H. Skov. 1989. Retrovirus-induced cell fusion is enhanced by protease treatment. J. Gen. Virol. 70, 1921-1927   DOI   ScienceOn
6 Chung, M., K. Kizhatil, L.M. Albritton, and G.N. Gaulton. 1999. Induction of syncytia by neuropathogenic murine leukemia viruses depends on receptor density, host cell determinants, and the intrinsic fusion potential of envelope protein. J. Virol. 73, 9377-9385
7 Jung, Y.T. and C.A. Kozak. 2003. Generation of novel syncytiuminducing and host range variants of ecotropic Moloney murine leukemia virus in Mus spicilegus. J. Virol. 77, 5065-5072   DOI
8 Pinter, A., T.-E. Chen, A. Lowey, N.G. Cortez, and S. Silagi. 1986. Ecotropic murine leukemia virus-induced fusion of murine cells. J. Virol. 57, 1048-1054
9 Wong, P.K.Y., P.H. Yuen, and S.J. Kaufman. 1977. Induction of syncytia by Moloney murine leukemia virus in myoblasts defective in differentiation. J. Virol. 21, 319-327
10 Davey, R.A., Y. Zuo, and J.M. Cunningham. 1999. Identification of a receptor-binding pocket on the envelope protein of Friend murine leukemia virus. J. Virol. 73, 3758-3763
11 Ou, W. and J. Silver. 2003. Role of a conserved amino-terminal sequence in the ecotropic MLV receptor mCAT1. Virology 308, 101-108   DOI   ScienceOn
12 Siess, D.C., S.L. Kozak, and D. Kabat. 1996. Exceptional fusogenicity of Chinese hamster ovary cells with murine retroviruses suggests roles for cellular factor(s) and receptor clusters in the membrane fusion process. J. Virol. 70, 3432-3439
13 Jones, J.S. and R. Risser. 1993. Cell fusion induced by the murine leukemia virus envelope glycoprotein. J. Virol. 67, 67-74
14 Germain, E., V.-G. Roulin, J. Qiao, P.O. de Campos Lima, and M. Caruso. 2005. RD114-pseudotyped retroviral vectors kill cancer cells by syncytium formation and enhance the cytotoxic effect of the TK/GCV gene therapy strategy. J. Gene Med. 7, 389-397   DOI   ScienceOn
15 Murphy, S.L., M. Chung-Landers, M. Honczarenko and G.N. Gaulton. 2006. Linkage of reduced receptor affinity and superinfection to pathogenesis of TR1.3 murine leukemia virus. J. Virol. 80, 4601-4609   DOI   ScienceOn
16 Park, B.H., B. Matuschke, E. Lavi, and G.N. Gaulton. 1994. A point mutation in the env gene of a murine leukemia virus induced syncytium formation and neurologic disease. J. Virol. 68, 7516- 7524
17 Albritton, L.M., J.W. Kim, L. Tseng, and J.M. Cunningham. 1993. Envelope-binding domain in the cationic amino acid transporter determines the host range of ecotropic murine retroviruses. J. Virol. 67, 2091-2096
18 Jung, Y.T., T. Wu, and C.A. Kozak. 2004. Novel host range and cytopathic variant of ecotropic Friend murine leukemia virus. J. Virol. 78, 12189-12197   DOI   ScienceOn