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Effect of Medium Components on the Lipstatin Production by Streptomyces toxytricini  

Lim, Mi-Ok (Faculty of Pharmacy, Yeungnam University)
Yin, Wencui (Faculty of Pharmacy, Yeungnam University)
Lee, Ji-Seon (Faculty of Pharmacy, Yeungnam University)
Yu, Yeon-Su (Faculty of Biotechnology, Yeungnam University)
Kim, Sang-Dal (Faculty of Biotechnology, Yeungnam University)
Nam, Doo-Hyun (Faculty of Pharmacy, Yeungnam University)
Publication Information
Korean Journal of Microbiology / v.42, no.3, 2006 , pp. 172-176 More about this Journal
Abstract
In order to increase the productivity of lipstatin by Steptomyces toxytricini, the effect of medium components on the lipstatin production was investigated. Using TSB medium as a basal medium, a variety of carbon sources, nitrogen sources, lipid and fatty acids was supplemented into a fermentation medium. The seed culture of S. toxytricini grown in 25 ml TSB medium at $28^{\circ}C$ for 3 days with agitation at 200 rpm was inoculated in the size of 2% in fermentation media containing different components and fermented at $28^{\circ}C$ for 60 more hrs. In the examination of the effect of carbon sources, the best cell growth was observed in fermentation media supplemented with glucose or glycerol, but the lipstatin productivity was the highest in media containing lactose or sucrose. Among complex nitrogen sources, yeast extract was the best one for cell growth, but the highest lipstatin production was found in TSB media composed of 1.7% casitone and 0.3% soytone. The increased concentration of triolein as a lipid caused the promotion of cell growth but the significant suppression of lipstatin production. When 0.5% fatty acids were supplemented to fermentation medium, unsaturated fatty acids like linoleic or oleic acid suppressed cell growth as well as lipstatin production, but 2 times higher lipstatin production was achieved by stearic acid, a saturated fatty acid, differently from expectation.
Keywords
fermentation; lipstatin; Streptomyces toxytricini;
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1 남두현. 1992. 방선균을 이용한 항생물질 발효. 미생물과 산업 18, 63-68
2 Aharonowitz, Y. 1980. Nitrogen metabolite regulation of antibiotic biosynthesis. Ann. Rev. Microbiol. 34, 209-233   DOI   ScienceOn
3 Demain, A.L., Y.M. Kennel, and Y. Aharonowitz. 1979. Carbon catabolite regulation of secondary metabusm, p.163-185. In A.T. Bull, D.C. Ellwood, and C. Ratledge (ed.) Microbial technology: current state, future prospects. Cambridge University Press, Cambridge
4 Imanaka, T., Y. Moriyama, G.G. Ecsedi, T. Aoyagi, K. Amanumamoto, S. Ohkuma, and T. Takano. 1983. Esterastin: A potent inhibitor of lysosomal acid lipase. J. Biochem. 94, 1017-1020   DOI
5 Eisenreich, W., E. Kupfer, W. Weber, and A. Bacher. 1997. Tracer studies with crude U-13C-lipid mixtures. Biosynthesis of the lipase inhibitor lipstatin. J. Biol. Chem. 272, 867-874   DOI   ScienceOn
6 Weibel, E.K., P. Hadvary, E. Hochuli, E. Kupfer, and H. Lengsfeld. 1987. Lipstatin, an inhibitor of pancreatic lipase, produced by Streptomyces toxytricini. I. Producing organism, fermentation, isolation and biological activity. J. Antibiot. 40, 1081-1085   DOI
7 Zhi, J., A.T. Melia, C. Funk, A. Viger-Chougnet, G. Hopfgartner, and B. Lausecker. 1996. Metabolic profiles of minimally absorbed orlistat in obese/overweight volunteers. J. Clin. Pharmacol. 36, 1006-1011   DOI   ScienceOn
8 Hochuli, E., E. Kupfer, R. Maurer, W. Meister, Y. Mercadal, and K. Schmidt. 1987. Lipstatin, an inhibitor of pancreatic lipase, produced by Streptomyces toxitricini. II. Chemistry and structure elucidation. J. Antibiot. 40, 1086-1091   DOI
9 Hollander, P.A., S.C. Elbein, I.B. Hirsch, D. Kelley, J. McGill, T. Taylor, S.R. Weiss, S.E. Crockett, R.A. Kaplan, J. Comstock, C.P. Lucas, P.A. Lodewick, W. Canovatchel, J. Chung, and I. Hauptman. 1998. Role of orlistat in the treatment of obese patients with type 2 diabetes. Diabetes Care 21, 1288-1294   DOI   ScienceOn
10 Goese, M., W. Eisenreich, E. Kupfer, W. Weber, and A. Bacher. 2000. Biosynthetic origin of hydrogen atoms in the lipase inhibitor lipstatin. J. Biol. Chem. 275, 21192-21196   DOI   ScienceOn
11 Martin, J.F. and A.L. Demain 1980. Control of antibiotic biosynthesis. Microbiol. Rev. 44, 230-251
12 Imanaka, T., K. Moto, S. Ohkuma, and T. Takano. 1981. Purification and properties of rabbit liver acid lipase (4-methylumbelliferyl oleate hydrolase). Biochim. Biophys. Acta. 665, 322-330   DOI   ScienceOn