Browse > Article

Typing of Extended-Spectrum ${\beta}$-Lactamase (ESBL) Producing Enterobacteriaceae Isolated from Slaughterhouse in Pusan, Korea  

Lee Hun-Ku (Department of Microbiology, Pukyong National Univ.)
Publication Information
Korean Journal of Microbiology / v.42, no.2, 2006 , pp. 125-130 More about this Journal
Abstract
The emergence of extended spectrum ${\beta}$-lactamase producing bacteria is causing very serious problems in Korea. Although there have been many reports about these bacteria Isolated from patients and clinical specimens, there is no report of extended spectrum ${\beta}$-lactamase producing organisms isolated from natural environment in Korea. This study was conducted to investigate the biological characteristics and extended spectrum ${\beta}$-lactamase types of eighteen strains of extended spectrum ${\beta}$-lactamase producing Klebsiella pneumoniae and Escherichia coli isolated from a slaughterhouse in Pusan in Korea during 2002 to 2004. Extended spectrum ${\beta}$-lactamases were identified by double-disk synergy test, conjugation, isoelectric focusing values and gene sequencing. Eight strains of Klebsiella pneumoniae and two strains of Eschericha coli were isolated kom pigs and transferred extended spectrum ${\beta}$-lactamase genes to recipient Escherichia coli J53 (sodium azide resistant and ceftazidime senstive) strain by conjugation. The conjugants of extended spectrum ${\beta}$-lactamase genes were alignments and translated to amino acids by BCM and NCBI blast. Eight conjugants of Klebsiella pneumonae were typed TEM-52, and two strains of Escherichia coli, SHV-12, but CMY-1 type were not detected in this study.
Keywords
extended-spectrum ${\beta}$-lactamase (ESBL); isoelectric focusing (IEF); Klebsiella pnuemoniae; Escherichia coli; TEM; SHV; CMY;
Citations & Related Records
연도 인용수 순위
  • Reference
1 이선화, 정재심, 이수연, 배현주, 나준, 박성종, 피수영, 배직현. 1997. Extended-spectrum ${\beta}-lactamase$ 를 생성하는 Klebsiella pneumoniae 패혈증 집단 발생의 분자역학조사. 병원감염관리. 2, 13-28
2 정윤섭, 이경원, 오까모도료이찌, 이노우에 마쓰히사. 1997. 임상검체에서 분리된 extended-spectrum ${\beta}-lactamase$항균제 분해 Klebsiella pneumoniae와 Escherichia coli 의 성상. 감염 29, 477-485
3 Billot-Klein, D., L. Gutmann, and E. Collatz. 1990. Nucleotide sequence of the SHV-5 ${\beta}-lactamase$ gene of a Klebsiella pneumoniae plasmid. Antimicrob. Agents Chemother. 34, 2439-2441   DOI   ScienceOn
4 Brinas, L., M. Zarazaga, Y. Saenz, F. Ruiz-Larrea and C. Torres. 2002. ${\beta}-lactamase$ in ampicillin-resistant Escherichia coli isolates from foods, humans, and healthy animals. Antimicrob. Agents Chemother. 46, 3156-3163   DOI
5 Bush, K., 1989. Characterization of ${\beta}-lactamase$. Antimicrob. Agents Chemother.. 33, 259-263   DOI   ScienceOn
6 Bush, K. and G. Jacoby. 1997. Nomenclature of TEM ${\beta}-lactamase$. J. Antimicrob. Chemother. 39, 1-3   DOI   ScienceOn
7 Jacoby, G.A. and P. Han. 1996. Detection of extended-spectrum ${\beta}-lactamase$ in clinical isolates of Klebsiella pneumoniae and Escherichia coli. J. Clin. Microbiol. 34, 908-911
8 Jacoby, G. A. and I. Carreras. 1990. Activities of ${\beta}-lactam $ antibiotics against Escherichia coli strains producing extended spectrum $\beta$-lactamases. Antimicrob. Agents Chemother. 34, 858-862   DOI   ScienceOn
9 Karen, B. and J. George. 1997. Nomenclature of TEM ${\beta}-lactamase$. Antimicrob. Agents Chemother 39, 1-3
10 Philippon, A., R. Labia, and G. Jacobi. 1989. Extended-spectrum ${\beta}-lactamase$ (minireview) Antimicrob. Agents Chemother. 33, 1131-1136   DOI   ScienceOn
11 Poirel, L., T. Naas, M. Guibert, E.B. Chaibi, R. Labia, and P. Nordmann. 1999. Molecular and Biochemical characterization of VEB-1 a novel class a extended spectrum ${\beta}-lactamase$ encoded by an Escherichia coli integron gene. Antimorob. Agents Chemother. 43, 573-581
12 Goussard, S. and P Courvalin. 1999. Update sequence information for TEM ${\beta}-lactamase$ gene. Antimicrob. Agents Chemother. 43, 367-370   DOI   ScienceOn
13 Pai, H. J., S. Lyu, J.H. Lee, J.M. Kim, Y.M. Kwon, J.W. Kim, and K.W. Choe. 1999. Survey of extended-spectrum ${\beta}-lactamase$ in clinical isolates of Escherichia coli and Klesbsiella pneumoniae : prevalence of TEM-52 in Korea. J. Clin. Microbiol. 37, 1758-1763
14 Ewing, W.H. 1986. Identification of Enterobacteriaceae. Elsevier, N. Y
15 이훈구. 2001. 광안리 오수처리장에서 분리된 extendedspectrum ${\beta}-lactamase$(ESBL) Klebsiella 와 Enterobacter 의 유형. 한국미생물학회지 37, 277-283
16 Meideiros, A.A. 1997. Evolution and dissemination of ${\beta}-lactamase$ accelerated by generation of ${\beta}-lactam $ antibiotics. Clin. Infect. Dis. 245, 19-45
17 배현주, 김정인, 권영미, 이경원, 정윤섭, 김의종, 조동택. 1997. 한국에서 분리된 Klebsiella pneumoniae가 생성하 는 extended-spectrum ${\beta}-lactamase$ 의 유형 및 특징. 감염 29, 93-103
18 이선화, 김미나, 최수진, 정화순. 2000. 임상 검체에서 분 리된 Escherichia coli 의 extended-spectrum ${\beta}-lactamase$ 성상. 대한임상병리학회지 20, 400-409
19 Matthew, M., R.W. Hedges, and J.T. Smith. 1979. Types of ${\beta}-lactamase$ determined by plasmid in gram negative bacteria. J. Bacteriol. 138, 657-662
20 Bagattini, M., V. Crivaro, A.D., Popolo, F. Gentile, A. Scarcella, M. Triassi, P. Villari and R. Zarilli. 2006. Molecular epidemiology of extended-spectrum (beta)-lactamase producing Klebsiella pneumonia in a neonatal intensive care unit. J. Antimicrobe. Chemother. 57, 979-982   DOI   ScienceOn
21 Nueesch-Inderbinen, M.T., Kayser, F.H., and Haechlier, H. 1997. Survey and molecular genetics of SHV ${\beta}-lactamase$ in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12. Antimicrob. Agents Chemother. 41, 943-949
22 Bush, K., G.A. Jacoby, and A.A. Medeiros. 1995. A functional classification scheme for ${\beta}-lactamase$ and its correlation with molecular structure. Antimicrob. Agents Chemother. 39, 1211- 1233   DOI   ScienceOn
23 김윤태, 이훈구. 2000. 부산시내 종합병원의 임상검체에 서 분리된 extended spectrum ${\beta}-lactamase$ 생성 Klebsiella pneumoniae의 형별분류. 한국미생물학회지 36, 221-227   과학기술학회마을
24 Medeiros, A.A. 1993. Nosocomial outbreaks of multi resistant bacteria extended spectrum ${\beta}-lactamase$ have arrived in north America. Ann. Inter. Med. 119, 428-430   DOI   ScienceOn
25 설성용, 장희경, 신행섭, 이유철 조동택. 김경숙 1998. 전달성 항균제 내성유전자의 분석에 의한 Serratia의 역학 조사. 대한 미생물학회지 33, 485-497
26 이경원, 조성란, 이창숙, 정윤섭, 권오현. 1994. Extended broad-spectrum ${\beta}-lactamase$ 생성 Escherichia coli 와 Klebsiella pneumoniae. 감염 26, 341-348
27 Ambuler, R.P., A.F.W. Coulson, N.M. Frere, J.M. Ghuysen, B. Joris, M. Forsman, R.C. Lebversque, G. Tiraby, and S.G. Waley. 1991. A standard numbering scheme for class A ${\beta}-lactamase$.. Biochem. J. 276, 269-272   DOI
28 Poyart, C., P. Mugnier, G. Quesum, P. Berche, and P. Trieu-Cout. 1998. A novel extended-spectrum TEM-type ${\beta}-lactamase$ (TEM- 52) associated with decreased susceptibility to moxalactam in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 42, 108- 113
29 National Committee for Clinical Laboratory Standards. 1998. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 4th ed. Pennsylvania: NCCLS: M7-A4
30 Winokur, P.L, A. Brueggemann, D.L. Desalvo, L. Hoffmann, M.D. Apley, E.K. Uhlenhopp, M.A. Pfaller, and G.V. Doern. 2000. Animal and human multidrug-resistant, cephalosporin-resistant Salmonella isolates expressing a plasmid-mediated CMY-2 AmpC ${\beta}-lactamase$. Antimicrob Agents Chemother 44, 2777-2783   DOI
31 Teshager, T., L. Dominguez, and M.A. Moreno. 2000. Isolaton of an SHV-12 ${\beta}-lactamase$producing Escherichia coli strain from a dog with recurrent urinary tract infections. Antimicrob. Agents Chemother. 44, 3483-3484   DOI
32 Miranda, G., N. Castro, and B. Leanos. 2004. Clonal and horizontal dissemination of Klebsiella pneumoniae expressing SHV-5 extended-spectum ${\beta}-lactamase$ in a Mexican pediatric hospital. J. Clin. Microbial. 42, 30-35   DOI
33 Sambrook, J., F.E. Fritsch, and T. Manilatis. 1989. Molecular cloning laboratory manual. Cold Spring Harbor Laboratory Press. I. 1.25-1.28
34 김병렬, 정석훈, 구자영, 이경원, 정윤섭, 정태전, 황현웅, 김미향. 1999. Extended- spectrum ${\beta}-lactamase$ 생성 장내 세균의 분리율 및 선별검사. 대한임상미생물학회지 2, 28-39
35 Rasheed, J.K., C, Jay, B. Metchock, F. Berkowitz, L. Wiegel, J. Crellin, C. Steward, B. Hill, A.A. Meideiros, and F.C. Tenover. 1997. Evoution of extended spectrum ${\beta}-lactam$ resistance (SHV-8) in a strain of Escherichia coli during multiple episodes of bacteremia. Antimicrob. Agents. Chemother. 41, 647-653