Browse > Article

PCR Cloning of Genes Encoding the Mn-Peroxidase Isozyme Family from Trametes versicolor KN9522 Using Degenerate Primers  

Jun, Sang-Cheol (Dept. of Biology, Division of Natural Science, Kangnung National University)
Kim, Kyu-Joong (Dept. of Biology, Division of Natural Science, Kangnung National University)
Publication Information
Korean Journal of Microbiology / v.42, no.1, 2006 , pp. 77-81 More about this Journal
Abstract
Degenerate primers corresponding to the sequences of the N-terminal regions of Mn-peroxidase isozymes were used to isolate the genomic fragments encoding the isozymes of Mn-peroxidase, CVMP1, CVMP2, CVMP3 and CVMP5 from the white-rot fungus Trametes versicolor KN9522. Three isozymes except one gave the expected PCR products (cmp1, cmp2 and cmp5) of about 900 base pairs, respectively. DNA sequence data obtained from each PCR products were used to analyze the BLAST program search on the National Center for Biotechnology Information. cmp1, cmp2 and cmp5 were similar to MPG-I (GenBank accession number Z30668) and PGV-II (GenBank accession number, Z54279) gene T. versicolor PRL572. PCR products of cmp1 and cmp2 showed 77%, 95% base sequence similarities to MPG-I gene and cmp5 showed about 88% similarity to PGV-II gene from T. versicolor PRL572. From this experiment, we could isolate genomic DNA fragments with degenerate primers designed from the N-terminal amino acid sequences of Mn-peroxidase isozyme family.
Keywords
degenerate primer; Mn-peroxidase; Trametes versicolor;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Johansson, T., and P.O. Nyman. 1993. Isozymes of lignin peroxidase and manganese(II) peroxidase from the white-rot basidiomycete Trametes versicolor : (I) Isolation of enzyme forms and characterization of physical and catalytic properties. Arch. Biochem. Biophys. 300, 49-56   DOI   ScienceOn
2 Johansson, T., and P.O. Nyman. 1996. A cluster of genes encoding major isozynes of lignin peroxidase and manganese peroxidase from the white-rot fungus Trametes versicolor. Gene 170, 31-38   DOI   ScienceOn
3 Lee, M.J., S.C. Jun, I.K. Hwang H.K. Choi, and K.J. Kim. 2005. Analysis of rDNA ITS region from Trametes spp. in Kangwon Province, Korea. Kor J. Mycol. 33(1), 1-10   과학기술학회마을   DOI
4 Zolan, M.E., and P.J. Pukkila. 1986. Inheritance of DNA methylation in Coprinus cinereus. Mol.Cell.Biol. 6, 185-200
5 Jonsson, L., and P.O. Nyman. 1994. A novel type of peroxidase gene from the white-rot fungus Trametes versicolor. Biochim. Biophys. Acta. 1207, 255-259   DOI   ScienceOn
6 Collins, P.J., M.M. O'brien and A.D.W. Dobson. 1999. Cloning and characterization of a cDNA encoding a novel extracellular peroxidase from Trametes versicolor. Appl. Environ. Microbiol. 65, 1343-1347
7 An, S., K.P. Park, H.T. Choi, K.J. Kim and K. Kim. 2003. Cloning of genomic DNAs of Trametes versicolor acting as autonomously replicating sequences in Saccharomyces cerevisiae. J. Microbiol. 40, 245-247
8 Johansson, T., K.G. Welinder, and P.O. Nyman. 1993. Isozymes of lignin peroxidase and manganese(II) peroxidase from the whiterot basidiomycete Trametes versicolor : (II) Partial sequences, peptide maps, and amino acid and carbohydrate compositions. Arch. Biochem. Biophys. 300, 57-62   DOI   ScienceOn
9 Jonsson, L., and P.O. Nyman. 1994. Tandem lignin peroxidase genes of the fungus Trametes versicolor. Biochim. Biophys. Acta. 1218, 408-412   DOI   ScienceOn
10 Irie,T., Y. Honda, H.C. Ha, T. Watanabe and M. Kuwahara. 2000, Isolation of cDNA and genomic fragments encoding the major manganese peroxidase isozyme from the white rot basidiomycete Pleurotus ostreatus. J. Wood Sci. 46, 230-233   DOI   ScienceOn
11 Kim, Y., S. Cheong, S. Kim, H. Song, and H. Choi. 2003. Partial cloning of genes for lignin degrading enzymes in Trametes versicolor. Kor. J. Microbiol. 39, 201-205   과학기술학회마을
12 Jun, S.C. 2004. Biochemical and molecular characterization of manganese peroxidase isozymes from Korean Trametes versicolor. PhD thesis, Kangnung National University
13 Kim, Y., S. Yeo, J. Kum, H.G. Song, and H.T. Choi. 2005. Cloning of a manganese peroxidase cDNA gene repressed by manganese in Trametes versicolor. J. Microbiol. 43, 569-571   과학기술학회마을