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Analysis of DNA Conformation in the Particles of Bacteriophage P4 Mutant, P4 ash8  

Song, Jae-Ho (Department of Life Science, College of Natural Science, Sunmoon University)
Kim, Kyoung-Jin (Department of Life Science, College of Natural Science, Sunmoon University)
Publication Information
Korean Journal of Microbiology / v.42, no.1, 2006 , pp. 62-66 More about this Journal
Abstract
To study the packaging mechanism of the bacteriophage P2-P4 system which is a useful experimental tool for the study of viral capsid assembly, we analyzed the DNA contents of P4 sid- mutant, P4 ash8 sid71's phage particles. Two kind of particles having different density were separated by the CsCl buoyant equilibrium density gradient experiment with fresh made stock of P4 ash8 sid71. The DNA from each particles was prepared and its conformations was analyzed by electrophoresis. Unexpectedly, both particles contain not only dimeric and trimeric but also monomeric P4 DNA.
Keywords
bacteriophage P2-P4; Escherichia coli; packaging; sid gene;
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