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Production of Lignin Degrading Enzymes and Decolorization of Various Dye Compounds by Wood-Rot Fungi  

Jang, Tae-Won (Dept of Biology, Kangnung National University)
Jun, Sang-Cheol (Dept of Biology, Kangnung National University)
Ahn, Tae-Seok (Dept of Environmental Science, Kangwon National University)
Kim, Kyu-Joong (Dept of Biology, Kangnung National University)
Publication Information
Korean Journal of Microbiology / v.42, no.1, 2006 , pp. 34-39 More about this Journal
Abstract
Wood-rot fungi produce extracellular lignin-degrading enzymes, the best known of which are lignin peroxidase, Mn-peroxidase and laccase. In this experiment, some of them produced all of three enzymes. Many other wood-rot fungi produced one or two of those enzymes with various combinations. In this experiment, we tried to clarify the relationship between the pattern of enzyme production and degradative activity of several dye compounds. From the 36 strains of 23 species of wood-rot fungi, Mn-peroxidase activity was found in 30 strains of the fungi tested, whereas the activity of lignin peroxidase and laccase was detected in 11 strains and 12 strains of species, repectively, in Kirks low nitrogen media. In relation to the activity of lignin degrading enzymes and degradation of dye compounds, the white-rot fungi with three kinds of enzymes tested showed the best dye decolorizers. The fungi with Mn-peroxidase activity only decolorized poly R-478 and remazol brilliant blue R dye in proportion to the enzyme activity, while methylene blue, bromophenol blue and congo red dye were degraded in regardless of enzyme activity. Those dyes were degraded in relation to the growth rate of mycelium. Brown-rot fungi did not degrade all the dye compounds except bromophenol blue, in spite of moderate growth rate.
Keywords
decolorization; dye compound; laccase; peroxidase; wood-rot fungi;
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