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Derepression of a Methionine Biosynthetic Gene by Utilizing a Promoter Isolated from Corynebacterium glutamicum  

Park Soo-Dong (Graduate School of Biotechnology, Korea University)
Park Ik-Hyun (Graduate School of Biotechnology, Korea University)
Choi Jong-Soo (BASF Research Center)
Kim Il-Kwon (BASF Research Center)
Kim Younhee (Department of Oriental Medicine, Semyung University)
Lee Heung-Shick (Department of Biotechnology, Korea University)
Publication Information
Korean Journal of Microbiology / v.41, no.4, 2005 , pp. 300-305 More about this Journal
Abstract
A transcriptionally active fragment $(P_{19})$ isolated by utilizing the promoter-probe shuttle vector pSK1Cat was analyzed. By subcloning analysis, the 180 bp region $(P_{180})$ responsible for the activity was determined. Transcriptional fusion of the C. glutamicum metX gene to $P_{180}\;(P_{180}-metX)$ resulted in a 24-fold increase in MetX activity in a complex medium, while a 13-fold increase was observed with the $P_{tac}$ promoter. Additionally, the expression conferred by $P_{180}$ was not affected by methionine added to the growth medium, suggesting that the $P_{180}$ clone is useful for the deregulated expression of biosynthetic genes in C. glutamicum during amino acid fermentation. Introduction of $P_{180}-metX$ into a lysine-producing C. glutamicum resulted in the production of methionine to 0.8 g/l.
Keywords
Corynebacterium glutamicum; homoserine acetyltransferase; metX; methionine; promoter;
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