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Cyanobacterial Diversity Analysis Using cpcBA-Intergenic Spacer Region  

Choi Gang-Guk (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
Park Yong-Ha (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
Ahn Chi-Yong (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Bae Myoung-Sook (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Oh Hee-Mock (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Microbiology / v.41, no.4, 2005 , pp. 287-292 More about this Journal
Abstract
The cyanobacterial diversity was analyzed by restriction fragment length polymorphism (RFLP) of PCR-amplified rpcBA-Intergenic Spacer (IGS) genes and cpcBA-IGS gene sequencing with a sample collected at Chuso-ri in Daechung Reservoir on March 15, 2005, The Shannon-Weiner diversity index was 0.65, indicating that the cyanobacterial community structure was simple. PCR-RFLP profiles obtained were Phormidium spp. (58 clones), Anabaena spp. (14 clones), Microcystis spp. (4 clones), Spirulina sp. (1 clone) and uncultured cyanobacteria (2 clones). The PCR-RFLP of cpcBA-IGS revealed that Phormidium spp. and Anabaena spp. dominated in the invested sample. As a consequence, it seems that the analysis of functional genes such as cpcBA-IGS can be used for the species identification and community analysis of cyanobacteria.
Keywords
community analysis; cpcBA-IGS; cyanobacteria; diversity; PCR-RFLP;
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