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Analysis of Microbial Community During the Anaerobic Dechlorination of Perchloroethylene and Trichloroethylene  

Lee Jae-Won (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Kim Byung-Hyuk (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Ahn Chi-Yong (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Kim Hee-Sik (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Yoon Byung-Dae (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Oh Hee-Mock (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Microbiology / v.41, no.4, 2005 , pp. 281-286 More about this Journal
Abstract
In this study, the anaerobic enrichment cultivation was performed with the sediments and the dredged soils from the cities of Ulsan, Masan, Yeosu, Gwangyang, Ansan and Seongnam. Acetate as an electron donor and PCE (perchloroethylene) or TCE (trichloroethylene) as an electron acceptor were injected into the serum bottle with an anaerobic medium. After the incubation of 12 weeks, the removal efficiency of PCE was highest at $70\%$ in the treatment with the sediment of Ulsan. Also, the bacterial community structure was analyzed by D-DGGE (double denatured gradient gel electrophoresis) through PCR-based 16S rDNA approaches. The dominant species id the anaerobic enrichment were found to belong to the genus of Desulfovibrio.
Keywords
DGGE (denaturing gradient gel electrophoresis); PCE; reductive dehalogena-tion; TCE;
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