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Proteome Profiling of Murine Macrophages Treated with the Anthrax Lethal Toxin  

Jung Kyoung-Hwa (Dept. of Biochemistry, Hanyang University)
Seo Giw-Moon (Dept. of Biochemistry, Hanyang University)
Kim Sung-Joo (Dept. of Biochemistry, Hanyang University)
Kim Ji-Chon (Dept. of Biochemistry, Hanyang University)
Oh Seon-Mi (Dept. of Biochemistry, Hanyang University)
Oh Kwang-Geun (Dept. of Bioprocess Technol., Biopolytech College)
Chai Young-Gyu (Dept. of Biochemistry, Hanyang University)
Publication Information
Korean Journal of Microbiology / v.41, no.4, 2005 , pp. 262-268 More about this Journal
Abstract
Intoxication of murine macrophages (RAW 264.7) with the anthrax lethal toxin (LeTx 100 ng/ml) results in profound alterations in the host cell gene expression. The role of LeTx in mediating these effects is unknown, largely due to the difficulty in identifying and assigning function to individual proteins. In this study, we have used two-dimensional polyacrylamide gel electrophoresis to analyze the protein profile of murine macrophages treated with the LeTx, and have coupled this to protein identification using MALDI-TOF mass spectrometry. Interpretation of the peptide mass fingerprint data has relied primarily on the ProFound database. Among the differentially expressed spots, cleaved mitogen-activated protein kinase kinase (Mek1) and glucose-6-phosphate dehydrogenase were increased in the LeTx treated macrophages. Mek1 acts as a negative element in the signal transduction pathway, and G6PD plays the role for the protection of the cells from the hyper-production of active oxygen. Our results suggest that this proteomic approach is a useful tool to study protein expression in intoxicated macrophages and will contribute to the identification of a putative substrate for LeTx.
Keywords
Anthrax lethal toxin; glucose-6-phosphate dehydrogenase (G6PD); mitogen-activated protein kinase kinase (Mek1); murine macrophages; proteomic techniques;
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