Browse > Article

Cloning of Acetate Kinase Gene from the Copepod Paracyclopina nana and its Expression in Escherichia coli  

Jung Sang-Oun (Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University)
Seo Jung Soo (Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University)
Lee Young-Mi (Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University)
Park Tae-Jin (Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University)
Kim Il-Chan (Polar BioCenter, Korea Polar Research Institute, Korea Ocean Research & Development Institute)
Park Heum Gi (Division of Marine Resource Development, College of Life Sciences, Kangnung National University)
Lee Jae-Seong (Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University)
Publication Information
Korean Journal of Microbiology / v.41, no.3, 2005 , pp. 157-163 More about this Journal
Abstract
The acetate kinase gene from the copepod Paracyclopina nana was cloned. The open reading frame (ORF) was 1,200 bp, and poly(A) signal sequence was located in the end of the ORF. After the molecular phylogenetic analysis of P nana acetate kinase gene, it was revealed that it formed the same branch with that of Aspergillus. Also P. nana acetate kinase showed the difference with those of other prokaryotic microorganisms but showed the same clade with those of fungi. We also confirmed that the recombinant protein of P. nana acetate kinase made approximately 50 kDa after expression of recombinant gene construct in E. coli. This may be useful to compare this protein to those of other organisms in biochemical characteristics.
Keywords
acetate kinase; Copepoda; Paracylopina nana; Phylogenetic analysis; recombinant protein;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Stern, J.R. and S. Ochoa. 1951. Enzymatic synthesis of citric acid. I. Synthesis with soluble enzymes. J. Biol. Chem. 191, 161-172   PUBMED
2 Kumar, S., K. Tamura, and M. Nei. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief. Bioinformat. 5, 150-163   DOI   ScienceOn
3 Aceti, D.J. and J.G. Ferry. 1988. Purification and characterization of acetate kinase from acetate-grown Methanosarcina thermophila. J. Biol. Chem. 263, 15444-15448   PUBMED
4 Nei, M. and S. Kumar. 2000. Molecular Evolution and Phylogenetics. Oxford University Press, New York
5 NCBI GenBank Search and Taxonomy Results
6 Romano, A.H. and W.J. Nickerson. 1954. Cystine reductase of pea seeds and yeasts. J. Biol. Chem. 208, 409-416   PUBMED
7 Reeves, R.E. and J.D. Guthrie. 1975. Acetate kinase (pyrophosphate). A fourth pyrophosphate-dependent kinase from Entamoeba histolytica. Biochem. Biophys. Res. Commun. 66, 1389-1395   DOI   ScienceOn
8 Romain, Y., S. Demassieux, and S. Carriere. 1982. Partial purification and characterization of two isoenzymes involved in the sulfurylation of catecholamines. Biochem. Biophys. Res. Commun.106, 999-1005   DOI   ScienceOn