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Identification of Bacterial Strains Adhered to Human Scalp Hair and Antimicrobial Susceptibility  

Lee Moon Sook (Department of Biology, Sunchon National University)
Han Hyo Shim (Department of Biology, Sunchon National University)
Jung Jae Sung (Department of Biology, Sunchon National University)
Publication Information
Korean Journal of Microbiology / v.41, no.1, 2005 , pp. 47-52 More about this Journal
Abstract
This study was carried out to identify bacterial strains adhered to human scalp hair and to investigate the antibiotic susceptibility of them. A total of 39 isolates were obtained from patients in intensive care units and healthy persons. The most common species isolated was Staphylococcus epidermidis (19 isolates), followed by S. aureus (14 isolates), S. waneri (5 isolates), and S. pasteuri (1 isolate). The susceptibility of isolates to amikacin, ampicillin, bacitracin, carbenicillin, cefazolin, cefoperazone, chloramphenicol, erythromycin, gentamicin, methicillin, nalidixic acid, neomycin, oxacillin, penicillin, streptomycin, tetracycline and vancomycin was determined by the disk diffusion method. All of the antibiotic resistant isolates were obtained from patient scalp hair. To examine the effect of conventional shampoo and detergent SDS on removing of bacteria from hair, we treated hair with culture solution of S. aureus. The bacteria attached to hair were not removed even by repeated washing with detergents. These results suggested that hair could be a source of bacterial contamination in hospital.
Keywords
antimicrobial susceptibility; cross-contamination; human scalp hair; Staphylococcus;
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1  Angeliki, P., J. Paparaskevas, S. Ioanna, and A. Athina. 2003. Changes in the epidemiology of methicillin-resistant Staphylococcus aureus in a Greek teriary care hospital, over an 8-year-period. Int. J. Antimicrob. Agents. 21, 542-546   DOI   ScienceOn
2  Assadian, O., F. Daxboeck, C. Aspeck, A. Blacky, R. Dunkl, and W. Koller. 2003. National surveillance of methicillin-sensitive and methcillin-resistant Staphylococcus aureus in Austrian hospitals: 1994-1998. J. Hosp. Infect. 55, 175-179   DOI   ScienceOn
3 Beasley, W.D., and G. Hirst. 2003. Making a meal of MRSA-the role of biosurgery in hospital-acquired infection. J. Hosp. Infect.55, 454-459
4 Boyce, J.M., G. Potter-Bynoe, C. Chenevert, and T. King. 1997. Environmental contamination due to methicillin-resistant Staphylococcus aureus: Possible infection control implications. Epidemiol. 18, 622-627
5  Chambers H.F. 1997. Methicillin resistance in Staphylococci: molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10, 781-791   PUBMED
6  Huijsmans-Evers, A.G.M. 1978. Results of routine tests for the detection of dispersers of Staphylococcus aureus. Arch. Chir. Neerl. 30, 141-150   PUBMED
7  Kloos, W.E., and T.L. Bannerman. 1994. Update on clinical significance of coagulase-negative staphylococci. Clin. Microbiol. Rev. 7, 117-140   DOI   PUBMED
8  Leclercq, R., C.J. Soussay, P. Weber, and N. Moniot-Ville. 2003. In vitro activity of the pristinamycin against the isolated staphylococci in the French hospitals in 1999-2000. Pathol. Biol. 51, 400-404   DOI   PUBMED   ScienceOn
9 Pfaller, M.A., and L.A. Herwaldt. 1998. Laboratory, clinical and epidemiological aspects of coagulase-negative staphylococci. Clin. Microbiol. Rev. 1, 281-299
10  Shiomori, T., H., Miyamoto, and K. Makishima. 2001, Significance of airborne transmission of methicillin-resistant Staphylococcus aureus in an otolaryngology head and neck surgery unit. Arch. Otolaryngol. Head Neck Surg. 127, 644-648   DOI   PUBMED
11 Takashi, K. 2003. Survey of methicillin-resistant coagulase negative staphylococci isolated from the fingers of nursing students. J. Infect. Chemother. 9, 30-34   DOI   ScienceOn
12 Teixeira, L.A., C.A. Resende, and L.R. Ormonde. 1995. Geographic spread of epidemic multiresistant Staphylococcus aureus clone in Brazil. J. Clin. Microbiol. 33, 2400-2404   PUBMED
13  Wang, J.T., Y.C. Chen, T.L. Yang, and S.C. Chang. 2002. Molecular epidemiology and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus in Taiwan. Diagnostic Microbiol. Infect. Dis. 42, 199-203   DOI   ScienceOn
14  Berger-Bachi B. 1997. Resistance not mediated by $\beta$-lactamase (methicillin resistance). p158-167. In Crossley K. B., Archer G. L., eds. The staphylococci in human disease. New York, Churchill Livingstone
15  Dominguez, M.A., H. Lencastre, J. Linares, and A. Tomasz. 1994. Spread and maintenance of a dominant methicillin resistant Staphylococcus aures (MRSA) clone during an outbreak of MRSA disease in a Spainish Hospital. J. Clin. Microbiol. 32, 2081-2087   PUBMED
16 Marrack, P., and J. Kappler. 1990. The staphylococcal enterotoxins and their relatives. Science 248, 705-711   DOI   PUBMED
17  Han, H.S., Y.J. Koh, J.-S. Hur, and J.S. Jung. 2003. Identification and characterization of coronatine-producing Pseudomonas syringae pv. actinidiae. J. Microbiol. Biotechnol. 13, 110-118
18  Deplano, A., A. Schuermans, J. Eldere, W. Witte, H. Meugnier, and J. Etienne. 2000. Multicenter evaluation of epidemiological typing of methicillin-resistant Staphylococcus aureus strains by repetitive element PCR analysis. J. Clin. Microbiol. 38, 3527-3533   PUBMED
19  Park, C.H., K.J. Choi, S.J. Moon, and C.S. Uhm. 2002. Structural development of scalp hair in children and effects of cosmetologic physico-chemical treatments on adult scalp hair. Kor. J. Electron. Microscop. 32, 339-344
20 Ruddy, M., M. Cummins, and Y. Drabu. 2001. Hospital hairdresser as a potential source of cross-infection with MRSA. J. Hosp. Infect. 49, 225-227   DOI   ScienceOn
21 McDonald, P., E. Mitchell, H. Johnson, and R. McDonnell. 2003. Epidemiology of MRSA: the North/South study of MRSA in Ireland 1999. J. Hosp. Infect. 54, 130-134   DOI   ScienceOn
22 Park, S.G., Y.O. Hwang, J.H. Jang, and K.M. Lee. 2001. Biological characteristics of Staphylococcus aureus isolated from food-born patients in Seoul. J. Food Hyg. Safety 16, 159-167
23  Silva-Coimbra, M.V., M.C. Silva-Carvalho, H. Wisplinghoff, G.O. Hall, and R.P. Wenzel. 2003. Clonal spread of methicillin resistant Staphylococcus aureus in a large geographic area of the United States. J. Hosp. Infect. 253, 103-110
24  Nakamura, R., N. Misawa, T. Oguri, and J. Igari. 1997. Incidence of coagulase negative staphylococci isolated from clinical materials, and drug resistance of isolates. J. Jpn. Clin. Microbiol. 7, 101-110
25  Bradley S.F. 1992. Methicillin-resistant Staphylococcus aureusinfection. Clin. Geriatr. Med. 8, 853-868   PUBMED
26  Moyer, C.L., F.C. Dobbs, and D.M. Karl. 1994. Estimation of diversity and community structure through restriction fragment length polymorphism distribution analysis of bacterial 16S rRNA genes from a microbial mat at an active, hydrothermal vent system, Loihi Seamount, Hawaii. Appl. Environ. Microbiol. 60, 871-879   PUBMED
27  Kiyomi, M., H. Toshinobu, H. Kazuto, K. Yasushi, Y. Takafumi, and O. Michio, 2000. Firm adherence of Staphylococcus aureusand Staphylococcus epidermidis to human hair and effect of detergent treatment. Microbiol. Immunol. 44, 453-656
28  Lizioli, A., G. Privitera, E. Alliata, E.M. Antonietta., and V. Carreri. 2003. Prevalence of nosocomial infection in Italy: result from the Lombardy survey in 2000. J. Hosp. Infect. 54, 114-148
29 Duckworth, G., B. Cookson, and H. Humphreys. 1998. Revised guidelines for the control of methicillin-resistant Staphylococcus aureus infection in hospitals. J. Hosp. Infect. 39, 253-290   DOI   ScienceOn
30 Takashi, I., R. Okamoto, C. Shimauchi, T. Okubo, A. Kuga, and M. Inoue. 2001. Identification of aminoglycoside-modifying enzymes by susceptibility testing: Epidemiology of methicillin-resistant Staphylococcus aureus in Japan. J. Clin. Microbiol. 39, 3115-3121   DOI   ScienceOn
31 Timothy, J.F., and H. Magnus. 1998. Surface protein adhesins of Staphylococcus aureus. Trend. Microbiol. 6, 484-488   DOI   ScienceOn
32 Patrick, C.C. 1990. Coagulase-negative staphylococci: pathogens with increasing clinical significance. J. Pediatrics 116, 497-507   DOI   PUBMED
33 Foster, T.J., and M. Hook. 1998. Surface protein adhesins of Staphylococcus aureus. Trends Microbiol. 6, 484-8   DOI   ScienceOn
34  Patti, J.M., B.L. Allen, M.J. McGavin, and M. Hook. 1994. MSCRAMM-mediated adherence of microorganisms to host tissues. Annu. Rev. Microbiol. 45, 585-617