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The Extracellular Enzyme Activities in Culture Broth of Sparassis crispa.  

Kim Ji-Young (동국대학교 대학원 생물학과)
Lim Chang-Soo (동국대학교 대학원 생물학과)
Kim Jae-Yong (동국대학교 대학원 생물학과)
Han Yeong-Hwan (동국대학교 대학원 생물학과)
Publication Information
Korean Journal of Microbiology / v.40, no.3, 2004 , pp. 230-231 More about this Journal
Abstract
The mycelia of Sparassis crispa DSMZ 5201 were cultivated at $24^{\circ}C$ for 15 days in yeast-malt extract-glucose broth (pH 4.0) and the filtrate was used as crude enzyme solution to determined the extracellular enzyme activity. The specific activity of $\alpha$-amylase was 44.27 unit/protein. The specific activities of protease, CMCase, $\beta$-glucosidase, chitinase, exo-$\beta$-l,4-glucanase were relatively high. However, a very little activity of xylanase was found.
Keywords
amylase; extracellular enzyme; saprotroph; Sparassis crispa;
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  • Reference
1 Brown, V. and G. Schmitz. 1980. Excretion of protease of Serratia marcescens. Arch. Microbiol. 124, 55-61
2 Min, E.G. and Y.H. Han. 2002. Optimal condition for mycelial growth of Beauveria bassiana and its extracellular enzyme activity. Kor. J. Microbiol. 38, 50-53
3 Shim, J.O., S.G. Son, S.O. Yoon, and Y.S. Lee. 1998. The optimal factors for the mycelial growth of Sparassis crispa. Kor. J. Mycol. 26, 39-46
4 Uriyo, M. and W.E. Eigel. 1999. Duration of killing treatment on $\alpha$-amylase, $\beta$-amylase and endo-(1,3)(1,4)-$\beta$-D-glucanase activity of malted sorghum (Sofghum bicolor). Process Biochemistry 35. 31-37
5 Lee, C.Y., O.P. Hong, M.J. Jung, and Y.H. Han. 1998. The extracellular enzyme activities in culture broth of Tricholoma matsutake. Kor. J. Mycol. 26, 496-501
6 Do, J.H. and S.D. Kim. 1985. Properties of amylase produced from higher fungi Ganoderma lucidum. Kor. J. Appl. Microbiol. Bioeng. 13, 173-178
7 Lee. J.H., C.S. Jung, and J.S. Cho. 2001. Purification and characterization of protease from Sarcodon aspratus (Berk.) S. Ito. Kor. J. Food Cookery Sci. 17, 497-502
8 Hsu, S.C. and L. Lockwood. 1975. Powdered chitin agar as a selective medium for enumeration of actinomycetes on water soil. Appl. Microbiol. 29, 422-426
9 Bradford, M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
10 Jeong E.U. and Y.H. Lee. 1995. Isolation of microorganism producing chitinase for chitooligosaccharides production, purification of chitinase, and its enzymatic characteristics. Kor. J. Appl. Microbiol. Biotechnol. 23, 187-196
11 Kanda, T., K. Wakabayashi, and K. Nisizawa. 1976. Purification and properties of and endocellulase of avicelase type from Irpex lacteus (Polyporus tuliferae). J. Ferment. Technol. 60, 381-383
12 Lee, S.S., J.K. Ha, and Y.J. Choi. 1996. Studies on the isolation and identification of rumen fungi, characterization of cellulolytic fungal enzymes, and its industrial utilization. Kor. J. Ankm. Nutr. Food 20, 51-63
13 Lee, T.H. and Y.H. Han. 2001. Enzyme activities of the fruit body of Ramaria botryis DGUM 29001. Kor. J. Mycol. 29, 173-175
14 Min, E.G. and Y.H. Han. 2000. Characteristics of extracellular $\beta$- glucosidase in Tricholoma matsutake. Kor. J. Biotech. Bioeng. 15, 9-13
15 Tokao, S., Y. Kamagata, and T. Sasaki. 1985. Cellulase production by Penicillum purpurogenum. J. Agri. Sci. Camb. 93, 217-222
16 Kim, D.J., H.J. Shin., B.H. Min, and K.H. Yoon. 1995. Isolation of a thermophilic Bacillus sp. producing the therm stable cellulasefree xylanase, and properties of the enzyme. Kor. J. Appl. Microbiol. Biotechnol. 23, 304-310
17 Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426-428