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Cloning and Characterization of Novel Cytochrome P450 Hydroxylase Genes from Pseudonocardia autotrophica  

Myeong Ji Seon (인하대학교 생물공학과)
Park Hyun-Joo (성원엔비켐㈜)
Han Kyuboem (㈜핸슨바이오텍)
Kim Sang-Nyun (㈜LG생활건강)
Kim Eung-Soo (인하대학교 생물공학과)
Publication Information
Korean Journal of Microbiology / v.40, no.3, 2004 , pp. 221-225 More about this Journal
Abstract
Novel cytochrome P450 hydroxylase (CYP) genes were isolated and characterized from P. autotrophica cosmid DNA library using an actinomycete CYP-specific PCR product as a screening probe. The cosmids containing four unique CYP genes (pESK601, 602, 603, 604, 605) were identified, and the four CYP genes were completely sequenced to be homologous to other known Actinomycetes CYP genes involved in various secondary metabolic pathways. Among all novel actinomycete CYP genes found in P. autotrophica, the CYP genes present in pESK601 were revealed to be highly homologous to the CYP genes involved in polyene-type amphotericin and nystatin antibiotic biosynthesis. The nucleotide sequences of the CYP flanking region in pESK601 also revealed the polyene-type biosynthetic genes, implying the presence of a cryptic polyene-type antifungal biosynthetic gene cluster in P. autotrophica.
Keywords
cytochrome P450 hydroxylase; polyene; Pseudonocardia autotrophica;
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