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Reidentification of Comamonas sp. Strain DJ-12 and Analysis of its pcbABC2D2 Genes Responsible for Degradation of 4-Chlorobiphenyl.  

이준훈 (충북대학교자연대미생물학과ㆍ바이오연구소)
박동우 (충북대학교자연대미생물학과ㆍ바이오연구)
강철희 (충북대학교자연대미생물학과ㆍ바이오연구)
채종찬 (충북대학교자연대미생물학과ㆍ바이오연구)
이동훈 (충북대학교자연대미생물학과ㆍ바이오연구)
김치경 (충북대학교자연대미생물학과ㆍ바이오연구소)
Publication Information
Korean Journal of Microbiology / v.40, no.2, 2004 , pp. 121-126 More about this Journal
Abstract
Comamonas sp. strain DJ-12 is a 4-chlobiphenyl(4CB)-degrading bacterium that was reidentified from Pseudomonas sp. DJ-12. The genomic DNA was isolated from the strain DJ-12 and amplified by PCR with primers for cloning pcbABCD genes responsible for degradation of 4CB. The amino acid sequences deduced from the nucleotide sequences of pcbA1, pcbA2, pcbA3, pcbA4, pcbB, pcbC2, and pcbD2 genes showed 91, 87, 99, 87, 97, 90 and 87％ homologies with those of Pseudomonas sp. KKS102, respectively. The pcbC1D1 genes that are involved in the degradation of (4-chloro)1,2-dihydroxybiphenyl produced from 4CB by pcbAB gene products were previously reported in the recombinant plasmid pCU1 from Pseudomonas sp. DJ-12. However, the pcbC2D2 genes in the plasmid pCT4 and pCT5 cloned from Comamonas sp. DJ-12 in this study showed 51 and 62％ homologies with those of pcbC1D1 in their nucleotide sequences. The pcbC1D1 and pcbC2D2 genes were found by Southern hybridization to be located at different loci on the chromosome of DJ-12 strain. These results indicate that Comamonas sp. strain DJ-12 has two different sets of pcbCD genes responsible for deg-radation of (4-chloro)1,2-dihydroxybiphenyl.
Keywords
4-chlorobiphenyl; Comamonas sp. DJ-12; degradation; pcbABCD;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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