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Immunoelectron Microscopic Localization and Analysis of Herpes simplex Virus Type 2 Antigens  

김천식 (서울아산병원 신경과)
오명환 (건국대학교 생명과학과)
Publication Information
Korean Journal of Microbiology / v.40, no.1, 2004 , pp. 23-28 More about this Journal
Abstract
Antigenic analysis of Herpes simplex type 2 virus was performed and its major antigen was localized using an immunoelectron microscopy. Antigens of 32, 43, 59 and 69 kDa were constantly expressed during the course of infection for 48 hr in the infected Vero cell. An antigen of 51 kDa was turned out to be the major one in inducing a immune response in Western-blot analysis. The 51 kDa antigen was localized on the surface of HSV-2 by immunoelectron microscopy using colloidal golds and anti-HSV 2 polyc1onal antibody. Immunofluorescence assay indicated that viral antigens were found throughout the infected cell and, especially, on the surface of the cell.
Keywords
Herpes simplex virus type 2; immunodominant antigen; immunofluorescence; immunogold labelling; localization;
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