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Cloning and Sequencing of Gene Fragment of Acid Proteinase from Penicillium oxalicum HCLF-34  

현성희 (을지의과대학교 자연과학교실)
천재순 (충북대학교 바이오연구)
강상순 (충북대학교 사범대)
김진규 (한국원자력연구소)
Publication Information
Korean Journal of Microbiology / v.40, no.1, 2004 , pp. 12-16 More about this Journal
Abstract
Acid proteinase has been discovered in Aspergillus niger (acid protease A) and Cryphonectria parasitica (acid proteinase EapC) and it plays major roles in cheese formation from milk. In this study, a partial gene encoding acid proteinase in Penicillium oxalicum HCLF-34 was cloned by using PCR with degenerate primers corresponding to highly conserved regions of the acid proteinase. The partial acid proteinase gene in P. oxalicum HCLF-34 contains an open reading frame of 438 base pairs and encodes an acid proteinase protein of 146 amino aicds. The predicted amino acid sequences showed 71 % homology with acid protease A and 67% homology with EapC.
Keywords
acid protease A; acid proteinase; EapC; P. oxalicum HCLF-34;
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