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Expression of Secretion-dedicated Srb Homologue and Antifungal Activity of Bacillus lentimorbus WJ5  

장유신 (한국원자력연구소 방사선이용연구부)
이영근 (한국원자력연구소 방사선이용연구부)
김재성 (한국원자력연구소 방사선이용연구부)
조규성 (한국원자력연구소 방사선이용연구부)
장병일 (한국원자력연구소 방사선이용연구부)
Publication Information
Korean Journal of Microbiology / v.39, no.3, 2003 , pp. 135-140 More about this Journal
Abstract
Bacillus sp. secretes high levels of extracellular enzymes into the culture medium. The signal recognition particle (SRP) and the SRP receptor play a central role in targeting pre secretory proteins to the translocase. By the analysis of the DNA microarray of B. lentimorbus WJ5, it was detected that WJ5m12, antifungal activity deficient mutant induced by gamma radiation, had a down-regulated expression of the SRP receptor gene (B. subtitis srb homologue, srbL). To determine the relationship of SRP receptor to antifungal activity, srbL of B. lentimorbus WJ5 was amplified by PCR and ligated into pQE30 vector, and then transferred into WJ5m12. The transformant, WJ5m12::srbL, recovered the antifungal activity. From the 2-DE analysis, the several presecretory proteins accumulated in the mutant cell and decreased to a level of the wild type in WJ5m12::srbL. It seems that the srbL could play an important role in the secretion of the antifungal activity related proteins of B. lentimorbus WJ5.
Keywords
antifungal activity; Bacillus lentimorbus WJ5; protein secretion; Srb;
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Times Cited By KSCI : 2  (Citation Analysis)
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