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Isolation and Characterization of Diesel Oil Degrading Bacterium, Pseudomonas sp. GENECO 1 Isolated from Oil Contaminated Soil  

이종광 (삼성엔지니어링 기술연구소)
김무훈 (삼성엔지니어링 기술연구소)
박형수 (삼성엔지니어링 기술연구소)
Publication Information
Korean Journal of Microbiology / v.39, no.2, 2003 , pp. 102-107 More about this Journal
Abstract
With the enrichment culture technique, bacterial strains which degrade diesel oil were isolated from soil contaminated with diesel oil. One of the isolates named GENECO 1 showed the highest activity for emulsification of diesel oil as well as the highest growth rate. This strain, GENECO 1, was identified as a Pseudomonas sp. based on its biochemical, physiological characteristics and 16S rDNA sequences. The optimal cultural conditions for cell growth and oil emulsifying activity of its culture were as follow; $30^{\circ}C$ for temperature, 7.0 for pH. Diesel oil degradation was analysed by the gas chromatography. More than 95% of 1% treated diesel oil were converted into a form no longer extractable by mixed organic solvents after 96 hours incubation.
Keywords
bioremediation; diesel oil; Pseudomonas sp.;
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