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Isolation and Identification of Bacteria Lysing Anabaena cylindrica  

Choi, Yong-Keel (Department of Life Science, Hanyang University, National Research Laboratory for Water Environmental & Restoration)
Hong, Yup (Department of Life Science, Hanyang University, National Research Laboratory for Water Environmental & Restoration)
Shin, Kyu-Chul (Department of Life Science, Hanyang University, National Research Laboratory for Water Environmental & Restoration)
Kim, Min-Seong (Department of Life Science, Hanyang University, National Research Laboratory for Water Environmental & Restoration)
Han, Myung-Soo (Department of Life Science, Environmental, Hanyang University, National Research Laboratory for Water Environmental & Restoration)
Publication Information
Korean Journal of Microbiology / v.38, no.2, 2002 , pp. 107-112 More about this Journal
Abstract
To isolate the bacteria lysing cyanobacteria, the sediment samples were collected from Dochang and Pal'tang Reservoir and Seokchon Lake. Each sample was smeared on the Anabaena cylindrica lawn and incubated in light chamber for 11 days. Bacteria having cyanobacteria-Iysing activity were isolated from the samples of Seokchon reservoir. Confirmation of cyanobacteria-Iysing activity was carried out to measure chlorophyll a and bacterial cell counting in mixed culture of Anabaena cylindrica and bacteria. Lysis was detected when extracellular meterials was added to the Anabaena cylindrica culture. The isolate was identified by analysis based on 16S rDNA sequence and morphological and physiological properties. The bacterial strain was taxonomically studied by the phylogenetic analysis based on 165 rDNA sequence. This strain was identified as a member of the genus Bacillus and designated as Bacillus sp. CHS1.
Keywords
16S rDNA; Anabaena cylindrica; Bacillus sp.;
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