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The Effect of Repetitive Magnetic Stimulation in an SCI Rat Model with Stem Cell Transplantation  

Bae, Young-Kyung (Department of Pathology, College of Medicine, Yeungnam University)
Park, Hea-Woon (Department of Rehabilitation Medicine, School of Medicine, Catholic University of Deagu)
Cho, Yun-Woo (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University)
Kim, Su-Jeong (Institute of Medical Science, Yeungnam University)
Lee, Joon-Ha (Department of Biochemistry and Molecular Biology, YeungnamUniversity)
Kwon, Jung-Gu (Department of Rehabilitation Medicine, School of Medicine, Catholic University of Deagu)
Ahn, Sang-Ho (Department of Pathology, College of Medicine, Yeungnam University)
Publication Information
The Journal of Korean Physical Therapy / v.22, no.1, 2010 , pp. 67-73 More about this Journal
Abstract
Purpose: We tested whether repetitive transcranial magnetic stimulation (rTMS) improved recovery following spinal cord injury (SCI) in rats with transplantation of adipose tissue-derived stromal cells (ATSCs). Methods: Twenty Sprague-Dawley rats (200-250 g, female) were used. Moderate spinal cord injury was induced at the T9 level by a New York University (NYU) impactor. The rat ATSCs (approximately $5{\times}10^5$ cells) were injected into the perilesional area at 9 days after SCI. Starting four days after transplantation, rTMS (25 Hz, 0.1 Tesla, pulse width=$370{\mu}s$, on/off time=3 sec/3 sec) was applied daily for 7 weeks. Functional recovery was assessed using the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale as well as pain responses for thermal and cold stimuli. Results: Both groups showed similar, gradual improvement of locomotor function. rTMS stimulation decreased thermal and cold hyperalgesia after 7 weeks, but sham stimulation did not. Conclusion: rTMS after transplantation of ATSCs in an SCI model may reduce thermal hyperalgesia and cold allodynia, and may be an adjuvant therapeutic tool for pain control after stem cell therapy in SCI.
Keywords
Repetitive transcranial magnetic stimulation; Adipose tissue-derived stromal cells; Locomotor function; Neuropathic pain; Spinal cord injury;
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