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http://dx.doi.org/10.12654/JCS.2019.35.4.08

Analysis of Cow Hide Glue Binder in Traditional Dancheong by Enzyme-linked Immunosorbent Assay  

Yu, Jia (Institute of Preservation Conservation of Cultural Properties, Korea National University of Cultural Heritage)
Chung, Yong Jae (Department of Heritage Conservation and Restoration, Graduate School of Cultural Heritage, Korea National University of Cultural Heritage)
Publication Information
Journal of Conservation Science / v.35, no.4, 2019 , pp. 363-372 More about this Journal
Abstract
Animal glue has been used as a binder in Dancheong since the Joseon dynasty. Binders play an important role in determining the physical characteristics of a painting layer. The analysis of binders can be used to identify the materials and techniques used in traditional Dancheong. Binders can be investigated using physicochemical component analyses methods such as gas chromatography/mass spectrometry, pyrolysis-gas chromatography/mass spectrometry, and fourier transform infrared spectroscopy, but the detection characteristics vary depending on the degradation properties of the pigment and binder. Therefore, cross-validation using a combination of physicochemical analysis and enzyme immunoassay is used to increase the reliability of the results. In this study, we present an enzyme-linked immunosorbent assay (ELISA) as an example of an enzyme immunoassay as a method for analyzing animal glue, a traditional binder used in Korea. The applicability of ELISA was tested using commercial animal glue, in addition to animal glue produced using a variety of extraction conditions. The animal glue was analyzed in a Noerok-additionally coated-replica sample to evaluate the possibility of analyzing the animal glue in a paint layer mixed with pigment. Based on the results, we performed an assay on the use of animal glue in the Dancheong sample of the temples of the Joseon dynasty, that are estimated to have been built in the 17th century.
Keywords
Dancheong; Binder; Animal glue; Enzyme-linked immunosorbent assay (ELISA);
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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1 Colombini, M.P. and Modugno, F., 2004, Characterisation of proteinaceous binders in artistic paintings by chromatographic techniques. Journal of Separation Science, 27(3), 147-160.   DOI
2 Cultural Heritage Administration, 2005, Seonunsa Daesoongjeon: actual survey report. 57-63. (in Korean)
3 Cultural Heritage Administration, 2016, Naesosa Daewoongbojeon dancheong: Pattern survey and copy. 41-54. (in Korean)
4 Hu, W., Zhang, H. and Zhang, B., 2015, Identification of organic binders in ancient Chinese paintings by immunological techniques. Microscopy and Microanalysis, 21(5), 1278-1287.   DOI
5 Kim, H.J., 2012, Understanding of Korean dancheong. Hantee Media, Seoul, 246-247. (in Korean)
6 Kim, S.K., Han, M.S., Jeong, S.H. and Kim, M.J., 2014, Study on the restored Sungnyemun Gate dancheong. Journal of Conservation Science, 30(4), 447-456. (in Korean with English abstract)   DOI
7 Koo, U.H., 2016, An experimental study for the reproduction of the dancheong technique for royal palaces since the 17th Century. Ph.D. dissertation, Myongji University, Yongin, 58-64. (in Korean with English abstract)
8 Korea National University of Cultural Heritage, 2018, Study on the manufacturing techniques and characteristics of traditional animal glue. 198-267. (in Korean)
9 Mazurek, J., Schilling, M., Chiari, G. and Heginbotham, A., 2008, Antibody assay to characterize binding media in paint. ICOM Committee for Conservation, New Delhi, September 22-26, 849-856.
10 Shinhanart, 1999, The science of colour paint(Han, B.L., Hwang, I.S. and Park, Y.B., trans.). Yekyung, Seoul, 26-27. (Original work published in 1994) (in Korean)
11 Song, Y.N, 2018, Materials characteristics of pigments on dancheong in Joseon dynasty. Ph.D. dissertation, Kongju University, Gongju, 126. (in Korean with English abstract)
12 Park, J.S., 2015, Analysis of animal glue by pyrolysis/GC/MS. Analytical Science & Technology, 28(3), 221-227. (in Korean with English abstract)   DOI
13 Park, J.S., Kim, M.J. and Kim, S.K., 2016, Confirming animal glue in dancheong sample by pyrolysis/GC/MS. Journal of Conservation Science, 32(1), 21-32. (in Korean with English abstract)   DOI
14 Ren, F., Atlasevich, N., Baade, B., Loike, J. and Arslanoglu, J., 2016, Influence of pigments and protein aging on protein identification in historically representative casein-based paints using enzyme-linked immunosorvent assay. Analytical and Bioanalytical Chemistry, 408(1), 203-215.   DOI
15 Yu, J.A., 2019, Analysis of binder in Korean traditional dancheong. Ph.D. dissertation, Korea National University of Cultural Heritage, Buyeo, 142-158. (in Korean with English abstract)