Browse > Article
http://dx.doi.org/10.5657/KFAS.2022.0067

Change in the Egg Diameter of Chub Mackerel Scomber japonicus Preserved in Fixing Solution  

Kim, So Ra (Coastal Water Fisheries Resources Research Division, National Institute of Fisheries Science)
Kim, Jung Jin (Coastal Water Fisheries Resources Research Division, National Institute of Fisheries Science)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.55, no.1, 2022 , pp. 67-72 More about this Journal
Abstract
We investigated the changes in the egg diameter of chub mackerel Scomber japonicus with the stages of egg development (and distinguished between hydrated oocyte and non-hydrated oocyte) for 1, 2, 3, 5, 10, 15 and 30 days. The chub mackerel oocytes were preserved in seven fixing solutions (70% ethyl alcohol, 99.9% ethyl alcohol, 5% formalin, 10% formalin, 5% neutral buffered formalin, 10% neutral buffered formalin and Gilson's solution). At 30 days, the chub mackerel hydrated oocytes preserved in 70% ethyl alcohol and 99.9% ethyl alcohol had shrunk by 5.2% and 7.9%, respectively. Similarly, the non-hydrated oocytes in the same solutions shrunk by 10.3% and 14.0%, respectively. Oocytes preserved in Gilson's solution had an average egg diameter decrease in both the hydrated oocyte (by 16.9%) and non-hydrated oocytes (by 15.6%). The diameter of the preserved hydrated oocytes did not significantly differ between the 5% formalin, 10% formalin, 5% neutral buffered formalin and 10% neutral buffered formalin, with shrinkage percentages of 0.6%, 0.1%, 1.9% and 3.4%, respectively (P>0.05). Similarly, the shrinkage percentages of the non-hydrated oocytes were 4.3% (5% formalin), 5.5% (10% formalin), 4.3% (5% neutral buffered formalin), and 4.1% (10% neutral buffered formalin).
Keywords
Egg diameter; Shrinkage; Preservation; Fixing solution; Scomber japonicus;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Shiraishi T, Ohta K, Yamaguchi A, Yoda M, Chuda H and Matsuyama M. 2005. Reproductive parameters of the chub mackerel Scomber japonicus estimated from human chorionic gonadotropin-induced final oocyte maturation and ovulation in captivity. Fish Sci 71, 531-542. https://doi.org/10.1111/j.1444-2906.2005.00997.x.   DOI
2 Hislop JRG and Bell MA. 1987. Observations on the size, dry weight and energy content of the eggs of some demersal fish species from British marine waters. J Fish Biol 31, 1-20. http://doi.org/10.1111/j.1095-8649.1987.tb05209.x.   DOI
3 Joseph J. 1963. Fecundity of yellowfin tuna (Thunnus albacares) and skipjack (Katsuwonus pelamis) from the eastern Pacific Ocean. Inter-Amer Trop Tuna Com Bull 7, 255-292.
4 Jung KM, Kang SK, Cha HK, Choi KH and Myksvoll MS. 2013. Buoyancy and vertical distribution of mackerel Scomber japonicus eggs in Korean waters. Korean J Fish Aquat Sci 46, 957-965. https://doi.org/10.5657/KFAS.2013.0957.   DOI
5 Kim SA and Zhang CI. 1994. Fish ecology. In: Spawning and early life history of fish. Kim JW, ed. Seoul Press, Seoul, Korea, 42-80.
6 Klibansky N and Juanes F. 2007. Species-specific effects of four preservative treatments on oocytes and ovarian material of Atlantic cod (Gadus morhua), haddock (Melanogrammus aeglefinus), and American plaice (Hippoglossoides platessoides). Fish Bull 105, 538-547.
7 Lubzens E, Young G, Bobe J and Cerda J. 2010. Oogenesis in teleosts: how fish eggs are formed. Gen Comp Endocrinol 165, 367-389. https://doi.org/10.1016/j.ygcen.2009.05.022.   DOI
8 Rakka M and Ganias K. 2015. Assessing species and stages-pecific effects of preservation on fish oocytes over different temporal scales. Mediterr Mar Sci 16, 533-537. https://doi.org/10.12681/mms.1205.   DOI
9 Yamada T, Aoki I and Mitani I. 1998. Spawning time, spawning frequency and fecundity of Japanese chub mackerel, Scomber japonicus in the waters around the Izu Island, Japan. Fish Res 38, 83-89. https://doi.org/10.1016/S0165-7836(98)00113-1.   DOI
10 Hostein I, Stock N, Soubeyran I, Marty M, De Mascarel I, Bui M, Geneste G, Petersen MC, Coindre JM and Macgrogan G. 2011. Nucleic acid quality preservation by an alcoholbased fixative: comparison with frozen tumors in routine pathology setting. Diagn Mol Pathol 20, 52-62. https://doi.org/10.1097/pdm.0b013e3181e71ba5.   DOI
11 Cha HK, Choi YM, Park JH, Kim JY and Sohn MH. 2002. Maturation and spawning of the chub mackerel, Scomber japonicus Houttuyn in Korean waters. J Korean Soc Fish Res 5, 24-33.
12 Dickerson TL, Macewicz BJ and Hunter JR. 1992. Spawning frequency and batch fecundity of chub mackerel, Scomber japonicus, during 1985. Cal Coop Ocean Fish Invest Rep 33, 130-140.
13 Lewis LA, Richardson DE, Zakharov EV and Hanner R. 2016. Integrating DNA barcoding of fish eggs into ichthyoplankton monitoring programs. Fish Bull 114, 153-165. https://doi.org/10.7755/fb.114.2.3.   DOI
14 Finn RN, Ostby GC, Norberg B and Fyhn HJ. 2002. In vivo oocyte hydration in Atlantic halibut (Hippoglossus hipoglossus): proteolytic liberation of free amino acids, and ion transport, are driving forces for osmotic water influx. J Exp Biol 205, 211-224. https://doi.org/10.1242/jeb.205.2.211.   DOI
15 Hajibabaei M, de Waard JR, Ivanova NV, Ratnasingham S, Dooh RT, Kirk SL, Mackie PM and Hebert PDN. 2005. Critical factors for assembling a high volume of DNA barcodes. Phil Trans R Soc B 360, 1959-1967. https://doi.org/10.1098/rstb.2005.1727.   DOI
16 Hunter JR, Lo NCH and Leong RJH. 1985. Batch fecundity in multiple spawning fishes. NOAA Technical Report NMFS 36, 67-77.
17 Karaiskou N, Triantafyllidis A, Alvarez P, Lopes P, Garcia-Vazquez E and Triantaphyllidis C. 2007. Horse mackerel egg identification using DNA methodology. Mar Ecol 28, 429-434. https://doi.org/10.1111/j.1439-0485.2007.00190.x.   DOI