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http://dx.doi.org/10.5657/KFAS.2021.0691

Development of a Simultaneous PCR Assay for Acute Hepatopancreatic Necrosis Disease (AHPND) and Enterocytozoon hepatopenaei (EHP) in Penaeus vannamei  

Jeon, Hye Jin (College of Veterinary Medicine, Kyungpook National University)
Lee, Chorong (College of Veterinary Medicine, Kyungpook National University)
Kim, Bum Keun (College of Veterinary Medicine, Kyungpook National University)
Kim, Sumi (Aquatic Disease Control Division, National Fishery Products Quality Management Service (NFQS))
Jang, Gwang Il (Aquatic Disease Control Division, National Fishery Products Quality Management Service (NFQS))
Rhee, Gahngyoon (Kunsan National University Department of Ocean Science and Engineering)
Kwon, Hyemin (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology)
Han, Jee Eun (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.54, no.5, 2021 , pp. 691-697 More about this Journal
Abstract
Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (VpAHPND) and Enterocytozoon hepatopenaei (EHP) are the two most important pathogens in shrimp aquaculture and they have caused enormous losses to the shrimp industry worldwide. In ponds, the major target organ for the two pathogens is the hepatopancreas, and infection with EHP is a known potential risk factor for VpAHPND infection. This study aimed to develop a PCR (polymerase chain reaction)-based diagnostic method for simultaneously detecting VpAHPND and EHP. The newly developed PCR diagnostic method could be used to test various samples, such as seawater, shrimp, and feces. The diagnostic method exhibited high sensitivity and specificity for both pathogens. This will help reduce the potential economic losses that may have been caused by the two major shrimp pathogens, VpAHPND and EHP, and will allow for the efforts and time spent combatting them to be dedicated elsewhere.
Keywords
Acute hepatopancreatic necrosis disease; Diagnosis; Enterocytozoon hepatopenaei; Peneaus (Litopenaeus) vannamei; Shrimp;
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