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http://dx.doi.org/10.5657/KFAS.2021.0660

Histopathological Examination of Myxosporean-Infected Olive Flounders Paralichthys olivaceus, Cultured in Jeju Island, South Korea  

Lee, Nam-Sil (Pathology Division, National Institute of Fisheries Science)
Kim, Aran (Pathology Division, National Institute of Fisheries Science)
Seo, Han-Gil (South Sea Fisheries Research Institute, National Institute of Fisheries Science)
Choi, He Sung (Pathology Division, National Institute of Fisheries Science)
Cho, Miyoung (Research Planning Division, National Institute of Fisheries Science)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.54, no.5, 2021 , pp. 660-667 More about this Journal
Abstract
In recent years, myxosporean infection from the cultured olive flounders Paralichthys olivaceus, have been frequently observed in Jeju island, South Korea. This study aimed to compare histopathological and molecular-biological methods of examining myxosporean infection from these flounders. Samples were obtained from affected individuals exhibiting emaciation or abdominal distention and a polymerase chain reaction (PCR) indicative of Parvicapsular anisocaudata, Enteromyxum leei and Kudoa septempunctata were initiated. Histopathological examination were conducted with H&E stained tissue sections, and then in-situ hybridization (ISH) reaction were processed with selected sections using P. anisocaudata, E. leei, K. septempunctata and Scuticociliate probes. Renal and intestinal tissue degeneration were common symptoms associated with all samples. Sever glomerular and renal tubular degeneration were evident, as were intestinal epithelial desquamation and spore formation in the epithelial cells. The results of conventional PCR analysis and ISH reactions revealed differences, and we suspect that various microparasites may have been associated with the symptoms manifested.
Keywords
Histopathology; ISH (in-situ hybridization); Myxosporean; Paralichthys olivaceus;
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