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http://dx.doi.org/10.5657/KFAS.2021.0152

Anti-Inflammatory Effect of Hexane Fraction from Eisenia bicyclis on Lipopolysaccharides-Treated RAW 264.7 Cells  

Kim, Bowoon (Department of Food Science and Nutrition, Pukyong National University)
Choi, Chang-Geun (Department of Ecological Engineering, Pukyong National University)
Kim, Jae-Il (Department of Food Science and Nutrition, Pukyong National University)
Kim, Hyeung-Rak (Department of Food Science and Nutrition, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.54, no.2, 2021 , pp. 152-161 More about this Journal
Abstract
Eisenia bicyclis is known to have secondary metabolites exhibiting various biological activities. In a preliminary study, the n-hexane fraction obtained from the ethanolic extract of E. bicyclis showed higher anti-inflammatory activity than the ethyl acetate and butyl alcohol fractions based on the inhibition of lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in RAW 264.7 cells. Using this fraction (E. bicyclis hexane fraction, EHF), we investigated the molecular mechanisms underlying its anti-inflammatory effect in LPS-stimulated RAW 264.7 cells. Pretreatment of the cells with up to 50 ㎍/mL EHF significantly inhibited NO and prostaglandin E2 production as well as their responsible enzyme proteins and mRNAs, in a dose-dependent manner (P<0.05). Similarly, EHF markedly reduced the production of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α as well as their mRNA levels. Nuclear translocation of nuclear factor-kappa B (NF-κB) was strongly suppressed by EHF treatment. EHF significantly reduced the phosphorylation of mitogen-activated protein kinases and phosphatidylinositol 3-kinase/Akt in LPS-stimulated cells. Moreover, EHF reduced ear edema in phorbol myristate acetate (PMA)-induced mice. These results indicate that EHF contains potent anti-inflammatory compounds, which may be used as a dietary supplement for the prevention of inflammatory diseases.
Keywords
Anti-inflammatory effect; Eisenia bicyclis; iNOS; $NF-{\kappa}B$; Pro-inflammatory cytokines;
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