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http://dx.doi.org/10.5657/KFAS.2020.0707

Inhibition of Lipid Accumulation in 3T3-L1 Adipocytes by Different Enzymatic Hydrolysates of Dried Red Sea Cucumber Stichopus japonicus  

Kim, Seo-Young (Chuncheon Center, Korea Basic Science Institute (KBSI))
Oh, Jae-Young (Department of Marine Life Science, Jeju National University)
Kim, Eun-A (Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST))
Heo, Soo-Jin (Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST))
Kim, Kil-Nam (Chuncheon Center, Korea Basic Science Institute (KBSI))
Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.53, no.5, 2020 , pp. 707-716 More about this Journal
Abstract
Red sea cucumber Stichopus japonicus, was dried using three methods-far-infrared ray, vacuum, and freeze drying and then enzymatically hydrolyzed using nine proteases: Alcalase, Flavourzyme, Kojizyme, Neutrase, Protamex, trypsin, α-chymotrypsin, and papain. In addition, the potential ability of hydrolysates to inhibit lipid accumulation in 3T3-L1 adipocytes was evaluated. The yield of hydrolysates from red sea cucumbers dried using each method was higher than that of the distilled water extract, and protein contents were either similar or higher. The hydrolysates that exhibited inhibitory effects on lipid accumulation, as demonstrated via Oil red O staining, were those obtained by far-infrared ray drying coupled with Alcalase, Flavourzyme, Kojizyme, or Neutrase treatment. In addition to the advantages of far-infrared drying and the characteristics of Flavourzyme, the Flavourzyme hydrolysate of far-infrared-dried red sea cucumber showed the highest inhibitory effect on lipid accumulation. In addition, this hydrolysate significantly decreased the expression of the protein factor fatty acid-binding protein 4, which is related to the late differentiation of 3T3-L1 adipocytes. Taken together, these results suggest that Flavourzyme hydrolysates from farinfrared-dried red sea cucumber may be used as a functional food and/or a pharmaceutical ingredient for the inhibition of lipid accumulation.
Keywords
Lipid accumulation; Adipocyte; Red sea cucumber; Far-infrared ray drying; Enzymatic hydrolysis;
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Times Cited By KSCI : 18  (Citation Analysis)
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1 Ding Y, Jiratchayamaethasakul C, Kim EA, Kim J, Heo SJ and Lee SH. 2018. Hyaluronidase inhibitory and antioxidant activities of enzymatic hydrolysate from Jeju Island red sea cucumber Stichopus japonicus for novel anti-aging cosmeceuticals. J Mar Biosci Biotechnol 10, 62-72. https://doi.org/10.15433/ksmb.2018.10.2.062.   DOI
2 Ko JY, Lee JH, Samarakoon K, Kim JS and Jeon YJ. 2013. Purification and determination of two novel antioxidant peptides from flounder fish Paralichthys olivaceus using digestive proteases. Food Chem Toxicol 52, 113-120. https://doi.org/10.1016/j.fct.2012.10.058.   DOI
3 Ko SC and Jeon YJ. 2015. Anti-inflammatory effect of enzymatic hydrolysates from Styela clava flesh tissue in lipopolysaccharide-stimulated RAW 264.7 macrophages and in vivo zebrafish model. Nutr Res Pract 9, 219-226. https://doi.org/10.4162/nrp.2015.9.3.219.   DOI
4 Ko SC, Lee JK, Byun HG, Lee SC and Jeon YJ. 2012. Purification and characterization of angiotensin I-converting enzyme inhibitory peptide from enzymatic hydrolysates of Styela clava flesh tissue. Process Biochem 47, 34-40. https://doi.org/10.1016/j.procbio.2011.10.005.   DOI
5 Lee HW, Lim NL, Cho K, Yang HY, Kim KJ, Kim MJ, Lee M, Kim DH, Koh HB, Jung WK, Roh SW and Kim D. 2014. Characterisation of inorganic elements and volatile organic compounds in the dried sea cucumber Stichopus japonicas. Food Chem 147, 34-41. https://doi.org/10.1016/j.foodchem.2013.09.110.   DOI
6 Lee JA, Park YJ, Jeong W, Hong SS and Ahn EK. 2017. Antiobesity effect of Amomum taso-ko ethanol extract in 3T3-L1 adipocytes. J Appl Bio Chem 60, 23-28. https://doi.org/10.3839/jabc.2017.005.   DOI
7 Lee MK, Kim SH, Ham SS, Lee SY, Chung CK, Kang IJ and Oh DH. 2000. The Effect of Far Infrared Ray-Vacuum Drying on the Quality Changes of Pimpinella bracycarpa. J Korean Soc Food Sci Nutr 29, 561-567.
8 Hwang DI, Choi IH, Kim DY, Park SM, Kim HB, Li YL and Lee HM. 2019. Inhibitory effects of Chrysanthemum boreale makino on 3T3-L1 preadipocyte differentiation and down-regulation of adipogenesis and lipogenesis. J Life Sci 29, 332-336. https://doi.org/10.5352/JLS.2019.29.3.332.   DOI
9 Je JG, Kim HS, Lee HG, Oh JY, Wang L, Rho S and Jeon YJ. 2019. Antioxidant and antihypertension effects of enzyme hydrolysate from Hippocampus abdominalis. Korean J Fish Aquat Sci 52, 127-133. https://doi.org/10.5657/KFAS.2019.0127.   DOI
10 Jeon MJ, Kim EJ, KIM GT, Kim GY, Lee SJ, Jung IC, Kim SY and Kim YM. 2018. Whitening effect and skin regeneration effect of red sea cucumber extract. J Life Sci 28, 681-687. https://doi.org/10.5352/JLS.2018.28.6.681.   DOI
11 Jeon MJ, Lim EG, Kim GY, Lee SJ, Jung IC, Kim SY and Kim YM. 2017. Anti-inflammatory effects and improving atopic dermatitis of ethanol extracts of red sea cucumber. Korean Soc Biotechnol Bioeng J 32, 335-341. https://doi.org/10.7841/ksbbj.2017.32.4.335.
12 Kim SW, Kim NS, Oh MJ, Kim HR, Kim MS, Lee DY, Yoon SH, Jung MY, Kim HJ, Lee CH and Oh CH. 2017. Antiobesity effects of fermented soybean oils in 3T3-L1 preadipocytes and high fat diet-fed C57BL/6J mice. J Korean Sco Food Sci Nutr. 46, 279-288. https://doi.org/10.3746/jkfn.2017.46.3.279.   DOI
13 Jun CI, Kim KH, Lee JY and Kim DG. 2004. Effect of velvet antler on the function of adipocytes (3T3-L1) and its association with IGF-1. J Pediatr Korean Med 18, 11-25.
14 Kim HS, Shin BO, Kim SY, Wang L, Lee WW, Kim YT, Rho S, Cho M and Jeon YJ. 2016. Antioxidant Activity of Pepsin Hydrolysate Derived from Edible Hippocampus abdominalis in vitro and in Zebrafish Models. Korean J Fish Aquat Sci 49, 445-453. https://doi.org/10.5657/KFAS.2016.0445.   DOI
15 Kim HY, Choi SJ, Ra HN and Lee JE. 2014. Antioxidative activities and quality characteristics of gruel as a home meal replacement with Angelica keiskei powder pre-treated by various drying methods. Korean J. Food Culture. 29, 91-100. https://doi.org/10.7318/KJFC/2014.29.1.091.   DOI
16 Park SY, Lim HK, Lee S, Hwang HC, Cho SK and Cho M. 2012. Pepsin-solubilised collagen (PSC) from Red Sea cucumber Stichopus japonicus regulates cell cycle and the fibronectin synthesis in HaCaT cell migration. Food Chem 123, 487-492. https://doi.org/10.1016/j.foodchem.2011.11.032.
17 Park SY, Lim HK, Park S and Cho M. 2012. Quality and preference changes red sea cucumber Stichopus japonicus Kimchi during storage period. J Appl Biol Chem 55, 135-140. https://doi.org/10.3839/jabc.2011.071.   DOI
18 Park JJ, Park DH and Lee WY. 2017. Quality change of sliced ginseng depending on different drying methods. Korean J Food Preserv 24, 361-366. https://doi.org/10.11002/kjfp.2017.24.3.361.   DOI
19 Saito H, Yamageta T and Suzuki S. 1968. Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates. J Biol Chem 243, 1536-1542.   DOI
20 Samarakoon K and Jeon YJ. 2012. Bio-functionalities of proteins derived from marine algae - A review. Food Res Int 48, 948-960. https://doi.org/10.1016/j.foodres.2012.03.013.   DOI
21 Seo EY. 2015. Effects of (6)-gingerol, ginger component on adipocyte development and differentiation in 3T3-L1. J Nutr Health 48, 327-334. https://doi.org/10.4163/jnh.2015.48.4.327.   DOI
22 Kim SY, Kim HS, Cho M and Jeon YJ. 2019. Enzymatic Hydrolysates of Hippocampus abdominalis regulates the skeletal muscle growth in C2C12 Cells and Zebrafish Model. J Aquat Food Prod Technol 28, 264-274. https://doi.org/10.1080/10498850.2019.1575940.   DOI
23 Liu X, Sun Z, Zhang M, Meng X, Xia X, Yuan W, Xue F and Liu C. 2012. Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicas. Carbohydr Polym 90, 1664-1670. https://doi.org/10.1016/j.carbpol.2012.07.047.   DOI
24 Chae HS, Yoo YM, Ahn CN, Cho SH, Park BY, Lee JM, Kim YK, Yun SG and Choi YI. 2003. Chemical and sensory characteristics of boiled soup extracted from crossbred ogol chicken as affected by the level of flavourzyme. Korean J Poult Sci 30, 11-16.
25 Choi J. 2017. Mechanistic evaluation on melanin biosynthesis inhibition in murine B16 melanoma byaqueousred sea cucumber Stichopus japonicus extracts. M.S. Thesis, Dongguk University, Seoul, Korea.
26 Kim SO and Jeung JS. 2019. Convergence study on the through inhibition of differentiation in 3T3-L1 cells of ethanol extract from Trichosanthes kirilowii Maxim. Root. J Korea Converg Soc 10, 127-133. https://doi.org/10.15207/JKCS.2019.10.3.127.   DOI