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http://dx.doi.org/10.5657/KFAS.2020.0688

Distribution and Molecular Characteristics of Vibrio vulnificus Isolated from Seawater Along the Gadeok Island Coast  

Oh, Hee-Kyung (Department of Industry 4.0 Convergence Bionics Engineering, Pukyong National University)
Jeong, Hee-Jin (Department of Food Science and Technology, Pukyong National University)
Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.53, no.5, 2020 , pp. 688-693 More about this Journal
Abstract
Vibrio vulnificus is a Gram-negative marine bacterium known to cause septicemia. This study was conducted to investigate the distribution of V. vulnificus along the coast of Gadeok Island in Korea and to determine the molecular characteristics of isolated strains sampled between March and November 2019 from seawater. The strains were mostly detected between July and September, when the average water temperature and average salinity were 22.2-26.2℃ and 14.2-29.9 psu, respectively. V. vulnificus was not detected in seawater below 15℃. In September, the highest population of V. vulnificus was observed at 2,100 MPN (most probable number)/100 mL, attributable to decreased salinity from heavy rains. In addition, the detection rate of V. vulnificus was higher at the sampling station near the Nakdong River. Virulence-related genes were also identified among the isolates, such as vvhA (97.1%), viuB (44.1%), and vcgC (57.4%). In particular, viuB and vcgC were only observed in V. vulnificus isolated from June to September, when the detection rate was high and water temperature was above 20℃, suggesting the role of seasonal characteristics.
Keywords
Vibrio vulnificus; Virulence gene; vvhA; viuB; vcgC;
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