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http://dx.doi.org/10.5657/KFAS.2020.0681

Isolation and Characterization of Polysaccharides Purified from Brown Alga Sargassum horneri  

Koo, Jae-Geun (Department of Food Science and Biotechnology, Kunsan National University)
Jeong, Seongwoo (Department of Food Science and Biotechnology, Kunsan National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.53, no.5, 2020 , pp. 681-687 More about this Journal
Abstract
Physicochemical properties of fucoidan and alginate extracted from Sargassium horneri were investigated. The alginates were extracted and purified via three different routes (CaCl2, HCl and ethanol routes), and their rheological properties at various concentrations and temperatures were determined. The yield of fucoidan was 3.08%, and it was mainly composed of fucose and galactose. Fourier-transform infrared spectrum of fucoidan showed strong absorption band at 1,254 and 827 cm-1 corresponding to the sulfate group. Flow behavior of the alginate solution was characterized using the power-law model. The consistency index increased with increasing concentrations. The sodium alginate solution (1.5%) exhibited Newtonian behavior when extracted via the CaCl2 and HCl routes, while it exhibited pseudoplastic behavior when extracted via the ethanol route. The effect of temperature on the flow behavior was investigated in terms of the activation energy (Ea), which was obtained using the Arrhenius equation. The Ea value corresponding to the alginate solution decreased with increasing shear rates. The Ea values corresponding to the alginate solutions purified via the CaCl2, HCl, and ethanol routes were 13.54-18.64 kJ/mol, 13.42-19.21 kJ/mol, and 9.51-10.67 kJ/mol, respectively. The low Ea values corresponding to the solutions extracted via the ethanol route suggest that the flow behavior does not depend significantly on temperature.
Keywords
Sagassium hornery; Fucoidan; Alginate; Viscosity; Fourier-transform infrared;
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