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http://dx.doi.org/10.5657/KFAS.2019.0605

Antimicrobial Resistance of Escherichia coli Isolated from Oysters Crassostrea gigas and Major Inland Pollution Sources in the Jaranman-Saryangdo Area in Korea  

Kwon, Ji Young (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science)
Kwon, Soon Jae (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science)
Yang, Ji Hye (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science)
Mok, Jong Soo (Food Safety and Processing Research Division, National Institute of Fisheries Science)
Jeong, Sang Hyeon (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science)
Ha, Kwang-Soo (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science)
Lee, Hee Jung (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science)
Jung, Yeoun Joong (Food Safety and Processing Research Division, National Institute of Fisheries Science)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.52, no.6, 2019 , pp. 605-616 More about this Journal
Abstract
This study evaluated the abundance and antimicrobial resistance of Escherichia coli in oysters Crassostrea gigas and major inland pollution sources sampled in 2014-2015 from Jaranman-Saryangdo on the southern coast of Korea. The ranges of the geometric mean of E. coli concentrations in oysters and major inland pollution sources were <20-29.8 MPN/100 g and 7.5-137.2 MPN/100 g, respectively. We isolated 247 strains of E. coli (28 from oysters and 247 from major inland pollution sources) and examined the antimicrobial resistance patterns of all isolates. Isolates from both sources were highly resistant to rifampin (99.5-100%) and cephalothin (70.8-78.6%). The resistance rate was higher in E. coli isolated from oysters those from inland pollution sources. Multiple resistance against at least four antimicrobials was observed in 85.7% and 21.0% of the oyster and major inland pollution sources isolates, respectively.
Keywords
Antimicrobial resistance; Oyster; Inland pollution source; Escherichia coli; Jaranman-Saryangdo;
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1 APHA (American Public Health Association). 1984. Laboratory procedures for the examination of seawater and shellfish. 5th Ed. American Public Health Association, Washington DC, U.S.A., 1-47.
2 Bosch AC, O'Neill B, Sigge GO, Kerwath SE and Hoffman LC. 2015. Heavy metal and marine fish meat and consumer health: a review. J Sci Food Agric 96, 32-48. https://doi.org/10.1002/jsfa.7360.   DOI
3 CLSI (Clinical and Laboratory Standards Institute). 2017. Performance standards for antimicrobial susceptibility testing. CLSI document M100-S26. Clinical and Laboratory Standards Institute. Wayne, PA, U.S.A., 29-76.
4 EC (European Commission). 2015. Commission regulation (EU) 2015/2285 amending Annex II to regulation (EC) No 854/2004 of the European rarliament and of the council. laying down specific rules for the organisation of official controls on products of animal origin intended for human consumption. Retrieved from http://data.europa.eu/eli/reg/2015/2285/oj on Oct 4, 2019.
5 Reinthaler FF, Ferierl G, Galler H, Haas D, Leitner E, Mascher F, Melkes A, Posch J, Winter I, Zarfel G and Marth E. 2010. ESBL-producing E. coli in Austrian sewage sludge. Water Res 44, 1981-1985. https://doi.org/10.1016/j.watres.2009.11.052.   DOI
6 Ryu AR, Park KBW, Kim SH, Ham IT, Ryu AR, Kwon JY, Kim JH, Yu HS, Lee HJ and Mok JS. 2017. Antimicrobial resistance patterns of Escherichia coli and Vibro parahaemolyticus isolated from shellfish from the southern coast of Korea. Korean J Fish Aquat Sci 50, 662-668. http://do.doi.org/10.5657/KFAS.2017.0662.   DOI
7 Son KT, Oh EG, Park KBW, Kwon JY, Lee HJ, Lee TS and Kim JH. 2009. Antimicrobial susceptibility of Escherichia coli isolated from fish farms on the southern coast of Korea. Korean J Fish Aquat Sci 42, 322-328. http://dx.doi.org/10.5657/kfas.2009.42.4.322.   DOI
8 Titilawo Y, Sibanda T, Obi L and Okoh A. 2015. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of water. Environ Sci Pollut Res 22, 10969-10980. http://doi.org/10.1002/jsfa.7360.   DOI
9 U.S. FDA (Food and Drug Administration). 2017. National shellfish sanitation program (NSSP), guide for the control of molluscan shellfish. Retrieved from http://www.fda.gov/media/117080/download on Oct 22, 2019.
10 WHO (World Health Organization). 2014. Antimicrobial resistance: global report on surveillance. Retrieved from http://www.who.int/drugresistance/documents/surveillancereport/en/ on Oct 4, 2019.
11 Jang YJ, Yoo YJ, Sul WJ, Cha CJ, Rhee OJ and Chae JC. 2017. Effect of antibiotic resistant factors in effluent of wastewater treatment plant on stream. Korean J Microbiol 53, 316-319. https://doi.org/10.7845/kjm.2017.7083.   DOI
12 Feldhusen F. 2000. The role of seafood in bacterial foodborne disease. Microbes Infect 2, 1651-1660. https://doi.org/10.1016/S1286-4579(00)01321-6.   DOI
13 Ferreira M, Vaz-Moreira I, Gonzalez-Pajuelo M, Nunes OC and Mannaia CM. 2007. Antimicirobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant. FEMS Microbiol Ecol 60, 166-176. https://doi.org/10.1111/j.1574-6941.2006.00268.x.   DOI
14 Grimes DJ. 1991. Ecology of estuarine bacteria capable of causing human disease: A review. Estuaries 14, 345-360. https://doi.org/10.2307/1352260.   DOI
15 Hunter C, Perkins J, Tranter J and Gunn J. 1999. Agricultural land-use effects on the indicator bacterial quality of an upland stream in the derbyshire peak district in the UK. Water Res 33, 3577-3586. https://doi.org/10.1016/S0043-1354(99)00083-4.   DOI
16 ISO (International Organization for Standardization). 2015. Microbiology of the food chain-Horizontal method for the enumeration of beta-gluccuronidase-positive Escherichia coli Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indoly-${\beta}$-D-glucuronide. International Organization for Standardization, Geneva, Switzerland.
17 Jo MR, Park YS, Park KBW, Kwon JY, Yu HS, Song KC, Lee HJ, Oh EG, Kim JH, Lee TS and Kim PH. 2016. Antimicrobial resistance in Escherichia coli isolated from shellfish farms on the west coast of Korea. Korean J Fish Aquat Sci 49, 13-19. https://dx.doi.org/10.5657/ KFAS.2016.0013.   DOI
18 KCDC (Korea Centers for Disease Control and Prevention) 2016. National action plan for combating antibiotic-resistance (2016-2020). Retrieved from https://www.cdc.go.kr/board.es?mid=a40303010000&bid= 0015 on Oct 22, 2019.
19 Kim JH, Park CK, Kim MY and Ahn SG. 2008. Contamination of veterinary antibiotics and antimicrobials in Han River basin. J Korean Soc Environ Anal 11, 109-118.
20 Kim JA, Park JH, Kim BY and Kim DS. 2017. The trend of acute respiratory tract infections and antibiotic prescription rates in outpatient settings using health insurance data. Korean J Clin Pharm 27, 186-194. https://doi.org/10.24304/kjcp.2017.27.3.186.   DOI
21 Kim SH, Kim KM, Kim GI and Choe JW. 2016a. Disinfection of E. coli from wastewater using non-contact type UV photoreactor and log inactivation index. J Korean Soc Water Wastewater 30, 1225-7672. http://dx.doi.org/10.11001/jksww.2016.30.2.139.
22 Kim YA, Park YS, Youk TM, Lee SS and Son YJ. 2016b. A study on the use of antibiotics in Korea and the resistance of major pathogens to antibiotics. National Health Insurance Service llsan hospital report 2016-20-001, NHIS Ilsan Hospital, Goyang, Korea, 1-116.
23 Krumperman PH. 1983. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol 46, 165-170.   DOI
24 Kummerer K. 2009. Antibiotics in the aquatic environment - A review-Part I. Chemosphere 75, 417-434. https://doi.org/10.1016/j.chemosphere.2008.11.086.   DOI
25 Kwon SJ, Jung YJ, Yoon HY, Mok JS and Kwon JY. 2018. Antimicrobial resistance in Escherichia coli isolated from oyster(Crassostrea gigas) and inland pollution sources in the Yongnam-Gwang-do area, Korea. Korean J Fish Aquat Sci 51, 673-681. http://do.doi.org/10.5657/ KFAS.2018.0673.   DOI
26 Levin BR, Lipsitch M, Perrot V, Schrag S, Antia R, Simonsen L, Walker NM and Stewart FM. 1997. The population genetics of antibiotic resistance. Clin Infect Dis 24, 9-16. https://doi.org/10.1093/clinids/24.supplement_1.s9.
27 Kwon SJ, Lee KJ, Jung YJ, Park SG, Go KR, Yang JH and Mok JS. 2016. Antimicrobial resistance of Escherichia coli isolates from mussel Mytilus galloprovincialis farms and inland pollution sources in the Changseon area, Korea. Korean J Fish Aquat Sci 49, 564-572. http://dx.doi.org/10.5657/KFAS.2016.0564.   DOI
28 Lee EJ, Park JH, Lee GW and Kim DS. 2019. The use of broad-spectrum antibiotics and antibiotics to treat antimicrobial-resistant bacteria. Yakhak Hoeji 63, 43-53. https://doi.org/10.17480/psk.2019.63.1.43.   DOI
29 Lee HJ, Kim HY, Kim KY, Yang DS, Lee IJ, Lim YK, Kim JH and Oh JE. 2017. Characteristic occurrence and distributions of pharmaceuticals in the Nakdong River. J Korean Soc Environ Eng 39, 403-411. https://doi.org/10.4491/KSEE.2017.39.7.403.   DOI
30 Lee HM, Yoon EJ, Kim DK, Jeong SH, Won EJ, Shin JH, Kim SH, Shin SH, Shin KS, Kim YA, Uh Y, Yang GW, Kim IH, Park C and Lee KJ. 2018. Antimicrobial resistance of major clinical pathogens in South Korea, May 2016 to April 2017: First one-year report from KOR-GLASS. Eurosurveillance 23, 1800047. https://doi.org/10.2807/1560-7917.ES.2018.23.42.1800047.
31 Mallin MA, Ensign SH, McIver MR, Shank GC and Fowler PK. 2001. Demographic, landscape, and meteorological factors controlling the microbial pollution of coastal waters. Hydrobiologia 460, 185-193. http://dx.doi.org/101023/A:1013169401211.   DOI
32 MFDS (Ministry of Food and Drug Safety). 2015. Report of the national antimicrobial use and resistance monitoringlivestock, meats and fishery products 2014. MFDS, Osong, Korea, 1-28.
33 Moura A, Araujo S, Alves MS, Henriques I, Pereira A and Correia ACM. 2014. The contribution of Escherichia coli from human and animal sources to the integron gene pool in coastal waters. Front Microbiol 419, 1-15. http://dx.doi.org/10.3389/fmicb.2014.00419.
34 MFDS (Ministry of Food and Drug Safety). 2019b. Report of the national antimicrobial use and resistance monitoringanimals, meats and fishery products 2018. MFDS, Osong, Korea, 1-35.
35 MOF (Ministry of Oceans and Fisheries). 2015. Sanitary criteria of producing, processing facilities and seawater area for fisheries product. Notice no. 2015-3, MOF, Sejong, Korea.
36 MOF (Ministry of Oceans and Fisheries). 2018. Sanitary criteria of Shellfish growing area. Notice no. 2018-166, MOF, Sejong, Korea.
37 NIER (National Institute of environmental research). 2011. A study of discharge source and variation for pharmaceuticals in the environment (IV). Retrieved from http://www.prism.go.kr/homepage on Oct 22, 2019.
38 Park KBW, Kim SH, Ham IT, Ryu AR, Kwon JY, Kim JH, Yu HS, Lee HJ and Mok JS. 2018. Antimicrobial resistance patterns of Escherichia coli isolated from discharged water from inland pollution sources in the Hansan-Heojeman and Jaranman-Saryangdo area of Korea. Korean J Fish Aquat Sci 51, 1-7. https://doi.org/10.5657/KAFS.2018.0001.   DOI
39 MFDS (Ministry of Food and Drug Safety). 2019a. Korea food code. Retrieved from http://www.foodsafetykorea.go.kr/foodcode/01_01.jsp on Oct 22, 2019.
40 MFDS (Ministry of Food and Drug Safety). 2016. Report of the national antimicrobial use and resistance monitoringlivestock and animal products 2015. MFDS, Osong, Korea, 1-37.
41 Peak N, Knapp CW, Yang RK, Hanfelt MM, Smith MS, Aga DS and Graham DW. 2007. Abundance of six tetracycline resistance genes in wastewater lagoons at cattle feedlots with different antibiotic use strategies. Environ Microbiol 9, 143-151. http://doi.org/10.1111/j.1462-2920.2006.01123.x.   DOI
42 Park KBW, Park JY, Jo MR, Yu HS, Lee HJ, Kim JH, Oh EG, Shin SB, Kim YK and Lee TS. 2013. Antimicrobial resistance in Escherichia coli isolated from shellfish farms in the southern coast of Korea. Korean J Fish Aquat Sci 46, 528-533. http://do.doi.org/10.5657/KFAS.2013.0528.   DOI