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http://dx.doi.org/10.5657/KFAS.2018.0397

Analysis of Erythromycin Resistance Gene in Pathogenic Bacteria Isolates from Cultured Olive flounder Paralichthys olivaceus in Jeju  

Lee, Da Won (School of Marine Biomedical Sciences, Jeju National University)
Jun, Lyu Jin (School of Marine Biomedical Sciences, Jeju National University)
Kim, Seung Min (Central Research Institute, Woo Gene B&G)
Jeong, Joon Bum (School of Marine Biomedical Sciences, Jeju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.51, no.4, 2018 , pp. 397-403 More about this Journal
Abstract
We determined the resistance rates of pathogenic bacteria isolated from cultured olive flounder Paralichthys olivaceus to erythromycin (Em), antibiotic typically used in aquaculture and analyzed the genotypes of resistant bacteria using polymerase chain reaction (PCR). We isolated and utilized 160 isolates of Streptococcus parauberis, 1 of S. iniae, 66 of Edwardsiella tarda, 56 of Vibrio sp. and 23 of unidentified bacteria from presumed infected olive flounder from Jeju Island from March 2016 to October 2017. Of the 306 isolated strains, Em-resistant strains included 33 of S. parauberis, 39 of E. tarda and 2 of Vibrio sp. We conducted PCR to assess the resistance determination of Em-resistant strains. Five different types of Em-resistance genes were detected in the 74 Em-resistant strains: erm (A), erm (B), erm (C), mef (A) and mef (E); erm (A) and erm (B) were detected in 1 (3%) and 24 (72.7%) S. parauberis isolates, respectively. In E. tarda, erm (B) was detected in five isolates (12.8 %) and no Em-resistance genes were detected in the two Vibrio sp. isolates.
Keywords
Erythromycin; Olive flounder; Resistance gene; Jeju; erm (B);
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