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http://dx.doi.org/10.5657/KFAS.2016.0678

Antibacterial Effect of Siegesbeckia pubescens Extract against Fish Pathogenic Streptococcus iniae  

Choi, Bo La (Department of Life Science and Biotechnology, Dong-eui University)
Cho, Eun-Ji (Department of Life Science and Biotechnology, Dong-eui University)
Lee, Myeong Jin (Department of Life Science and Biotechnology, Dong-eui University)
Lee, Seong Hyun (Department of Life Science and Biotechnology, Dong-eui University)
Kim, Chae Eun (Department of Life Science and Biotechnology, Dong-eui University)
Oh, Se Young (Department of Life Science and Biotechnology, Dong-eui University)
Kim, Kyoung-Hoon (JNS Tech Co.)
Jeong, Chang Hwa (JNS Tech Co.)
Lim, Eun Seo (Department of Food Science and Nutrition, Tongmyong University)
Kim, Tae Hoon (Department of Food Science and Biotechnology, Daegu University)
Lee, Eun-Woo (Department of Life Science and Biotechnology, Dong-eui University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.49, no.5, 2016 , pp. 678-682 More about this Journal
Abstract
Antibacterial activity of 80% methanol extract from 13 commercial herb medicines was measured against fish pathogens Streptococcus iniae, causing several diseases in various kind of fish. Siegesbeckia pubescens showed the strongest antibacterial activity against S. iniae. Methanol extract of S. pubescens was further extracted using several organic solvents having different polarity. Extract from n-hexane and ethyl acetate fraction showed strong activity. Minimal inhibitory concentration, MIC of S. pubescens extract was measured and resulted showing 8 μg/mL with n-hexane fraction and 32 μg/mL with ethyl acetate fraction against S. iniae. The growth of S. iniae was fully inhibited by adding 50 μg/mL (final concentration) of n-hexane or ethyl acetate fraction in the liquid media. It is needed that, from these results, purification and isolation of responsible active compound(s) of these activities and further study on the synergy effect using combination with commercial antibiotics against fish pathogenic bacteria.
Keywords
Siegesbeckia pubescens; Antibacterial activity; Streptococcus iniae;
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Times Cited By KSCI : 7  (Citation Analysis)
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