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http://dx.doi.org/10.5657/KFAS.2016.0628

Development of Allotriploid Embryos from Female Olive Flounder Paralichthys olivaceus Crossed with Male Starry Flounder Platichthys stellatus  

Jung, Hyo Sun (Department of Fisheries Biology, Pukyong National University)
Ko, Min Gyun (Department of Fisheries Biology, Pukyong National University)
Lee, Hyo Bin (Department of Fisheries Biology, Pukyong National University)
Kim, Dong Soo (Department of Marine Bio-Materials & Aquaculture, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.49, no.5, 2016 , pp. 628-634 More about this Journal
Abstract
We investigated the characteristics and rate of development of allotriploid embryos derived from a cross between female olive flounder Paralichthys olivaceus and male starry flounder Platichthys stellatus. The allotriploidy was induced by cold shocking fertilized eggs three minutes post-fertilization at 3°C for 45 minutes. The average cellular DNA content of the allotriploid embryos was 2.06±0.03 pg/cell, which is equal to the sum of the cellular DNA content of a diploid olive flounder (1.42 pg/cell) and a haploid starry flounder (0.66 pg/haploid cell). The first cleavage, midblastula, gastrula and Kupffer's vesicle appearance stages of the allotriploid eggs began at 1.5, 8, 13 and 26 hours after cold shocking at 18°C, respectively. The developmental rate of allotriploid eggs was equivalent to that of diploid and triploid olive flounder eggs at 10, 14 and 18°C. However, the hatching times of allotriploid eggs, 7 h at 10°C, 5 h at 14°C and 4 h at 18°C, were earlier than those of diploid and triploid olive flounder.
Keywords
Embryonic development; Allotriploid; Olive flounder;
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Times Cited By KSCI : 5  (Citation Analysis)
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