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http://dx.doi.org/10.5657/KFAS.2016.0594

Anti-HIV-1 Activity of Gelatin Hydrolysate Derived from Alaska Pollack Theragra chalcogramma Skin  

Park, Sun-Joo (Department of Chemistry, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.49, no.5, 2016 , pp. 594-599 More about this Journal
Abstract
Infection with HIV (Human immunodeficiency virus), over time, develops into acquired immunodeficiency syndrome (AIDS). The development of non-toxic and effective anti-HIV drugs is one of the most promising strategies for the treatment of AIDS. In this study, we investigated the anti-HIV-1 activity of gelatin hydrolysates from Alaska pollack skin. Gelatin hydrolysates were prepared using four enzymes (alcalase, flavourzyme, neutrase, and pronase E). Among these, the pronase E gelatin hydrolysate was found to inhibit HIV-1 infection in the human T cell-line MT4. It exhibited inhibitory activity on HIV-1IIIB-induced cell lysis, reverse transcriptase activity, and viral p24 production at noncytotoxic concentrations. Moreover, it decreased the activation of matrix metalloproteinase-2 (MMP-2) in vitro. Because HIV infection-induced activation of MMP-2 can accelerate collagen resolution and collapse of the immune system, pronase E gelatin hydrolysate might prevent the activation of MMP-2 in cells, resulting in collagen stabilization and immune cell homeostasis consistent with anti-HIV activation. These results suggest that pronase E gelatin hydrolysate could potentially be incorporated into a novel therapeutic agent for HIV/AIDS patients.
Keywords
AIDS; Anti-HIV-1 activity; Alaska Pollack skin; Gelatin; Enzymatic hydrolysate;
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Times Cited By KSCI : 2  (Citation Analysis)
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