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http://dx.doi.org/10.5657/KFAS.2016.0045

Changes in Respiratory Metabolism and Blood Chemistry of Olive Flounder Paralichthys olivaceus Exposed to Hypoxia  

Han, Ji-Do (Danyang Danuri Center)
Kim, Heung-Yun (Department of Aqualife Medicine, Chonnam National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.49, no.1, 2016 , pp. 45-52 More about this Journal
Abstract
This experiment investigated changes in metabolic rate (MO2), critical oxygen saturation (Scrit), and blood chemistry of olive flounder Paralichthys olivaceus exposed to progressive hypoxia and returned to normoxic water at 20°C. The normoxic standard metabolic rate (SMR) and routine metabolic rate (RMR) were 69.5-83.9 and 70.2-156.4 mg O2 kg-1h-1, respectively based on fish weight. Scrit was 31.0% dissolved oxygen (DO) at 20°C. After returning the fish to 70% DO following exposure to hypoxia (20% DO), MO2 increased two-fold compared to the normoxic SMR and then decreased into the range of the RMR with time. Blood PO2 and plasma lactate decreased significantly after exposure to hypoxia (20% DO) and then increased as ambient oxygen saturation decreased. Cortisol levels increased as ambient oxygen saturation decreased, but the levels decreased rapidly in the range of the normoxic control when the fish were returned to ambient water with 70% DO. Plasma glucose levels increased when the fish were returned to normoxic water after exposure to a progressively more hypoxic condition.
Keywords
Olive flounder; Paralichthys olivaceus; Hypoxia; Respiratory metabolism; Blood chemistry;
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  • Reference
1 Barton BA and Iwama GK. 1991. Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu Rev Fish Dis 1, 3-26.   DOI
2 Jobling M. 1982. A study of some factors affecting rates of oxygen consumption of plaice, Pleuronectes platessa L. J Fish Biol 20, 501–516.   DOI
3 Dalla Via J, Van Den Thillart G, Cattani O and De Zwaan A. 1994. Influence of long-term hypoxia exposure on the energy metabolism of Solea solea. II. Intermediary metabolism in blood, liver and muscle. Mar Ecol Prog Ser 111, 17-27.   DOI
4 Fry FEJ. 1971. The effect of environmental factors on the physiology of fish. In : Fish Physiology. Hoar WS & Randall DJ eds. Academic Press, New York, U.S.A, 1-98.
5 Fu SJ, Xie XJ and Cao ZD. 2005. Effect of feeding level and feeding frequency on specific dynamic action in Silurus meridionalis. J Fish Biol 67, 171-181. http://dx.doi.org/10.1111/j.0022-1112.2005.00722.x.   DOI
6 Herbert NA and Steffensen JF. 2005. The response of Atlantic cod, Gadus morhua, to progressive hypoxia: fish swimming speed and physiological stress. Mar Biol 147, 1403-1412.   DOI
7 Johansen JL, Herbert NA and Steffensen JF. 2006. The behavioural and physiological response of Atlantic cod Gadus morhua L. to short-term acute hypoxia. J Fish Biol 68, 1918-1924. http://dx.doi.org/10.1111/j.1095-8649.2006.01080.x.   DOI
8 Jorgensen JB and Mustafa T. 1980. The effect of hypoxia on carbohydrate metabolism in flounder (Platichthys flesus)-I. Utilization of glycogen and accumulation of glycolytic end products in various tissues. Comp Biochem Physiol B 67, 243-248.   DOI
9 Lennard R and Huddart H. 1992. The effects of hypoxic stress on the fine structure of the flounder heart (Platichthys flesus). Comp Biochem Physiol A Physiol 101, 723-732.   DOI
10 Maxime V, Pichavant K, Boeuf G and Nonnotte G. 2000. Effect of hypoxia on respiratory physiology of turbot, Scophthalmus maximus. Fish Physiol Biochem 22, 51-59.   DOI
11 Poon WL, Hung CY and Randall DJ. 2001. The effect of aquatic hypoxia on fish. In: Proceedings of the Sixth International Symposium on Fish Physiology, Toxicology and Water Quality. Thurston RV, eds. Ecosystems Research Division, Georgia, 31-49.
12 Miller DC, Poucher SL and Coiro L. 2002. Determination of lethal dissolved oxygen levels for selected marine and estuarine fishes, crustaceans, and a bivalve. Mar Biol 140, 287-296. http://dx.doi.org/10.1007/s002270100702.   DOI
13 Noga EJ. 1999. Problems 1 through 9. In: Fish disease: diagnosis and treatment. Iowa State University Press, U.S.A., 55-74.
14 Peck MA, Buckley LJ and Bengtson DA. 2005. Effects of temperature, body size and feeding on rates of metabolism in young of the year haddock. J Fish Biol 66, 911-923. http://dx.doi.org/10.1111/j.0022-1112.2005.00633.x.   DOI
15 Robertson L, Thomas P, Arnold CR and Trant JM. 1987. Plasma cortisol and secondary stress responses of red drum to handling, transport, rearing density, and disease outbreak. Prog Fish-Cult 49, 1-12.   DOI
16 Priede IG. 1985. Metabolic scope in fishes. In: Fish energetics: new perspectives. Tytler P and Calow P, eds. Johns Hopkins University Press, Baltimore, U.S.A., 33-64.
17 Randall D. 1982. The control of respiration and circulation in fish during exercise and hypoxia. J Exp Biol 100, 275-288.
18 Richards JG. 2009. Metabolic and molecular responses of fish to hypoxia. In: Hypoxia. Richards JG, Farrell AP and Brauner CJ, eds. Academy Press, London, U.K., 443-485.
19 Schurmann H and Steffensen JF. 1997. Effects of temperature, hypoxia and activity on the metabolism of juvenile Atlantic cod. J Fish Biol 50, 1166-1180.
20 Soldatov AA. 1996. The effect of hypoxia on red blood cells of flounder: a morphologic and autoradiographic study. J Fish Biol 48, 321-328.   DOI
21 Steffensen JF. 2006. Oxygen consumption of fish exposed to hypoxia: Are they all oxyregulators or are any oxyconformers?. Proceedings of the 9th International Symposium on Fish Physiology, Toxicology and Water Quality. Brouner CJ, Suvajdzic K, Nilsson G and Randall D, eds. Ecosystems Research Division, Georgia, 239-250.
22 Steffensen JF. 1989. Some errors in respirometry of aquatic breathers: how to avoid and correct for them. Fish Physiol Biochem 6, 49-59.   DOI
23 Steffensen JF, Lomholt JP and Johansen K. 1982. Gill ventilation and O2 extraction during graded hypoxia in two ecologically distinct species of flatfish, the flounder (Platichthys flesus) and the plaice (Pleuronectes platessa). Environ Biol Fish 7, 157-163.   DOI
24 Wendelaar Bonga SE. 1997. The stress response in fish. Physiol Rev 77, 591-625.   DOI
25 Tallqvist M, Sandberg-Kilpi E and Bonsdorff E. 1999. Juvenile flounder, Platichthys flesus (L.), under hypoxia: Effects on tolerance, ventilation rate and predation efficiency. J Exp Mar Biol Ecol 242, 75-93.   DOI
26 Thuy NH, Tien LA, Tuyet PN, Huong DTT, Cong NV, Bayley M, Wang T and Lefevre S. 2010. Critical oxygen tension increases during digestion in the perch Perca fluviatilis. J Fish Biol 76, 1025-1031. http://dx.doi.org/10.1111/j.1095-8649.2009.02533.x.   DOI
27 Van Den Thillart G, Dalla Via J, Vitali G and Cortesi P. 1994. Influence of long-term hypoxia exposure on the energy metabolism of Solea solea. I. Critical O2 levels for aerobic and anaerobic metabolism. Mar Ecol Prog Ser 104, 109-117.   DOI
28 White A and Fletcher TC. 1989. The effect of physical disturbance, hypoxia and stress hormones on serum components of the plaice, Pleuronectes platessa L. Comp Biochem Physiol A 93, 455-461.   DOI
29 Zar JH. 1984. Biostatistical Analysis. 2nd ed., Prentice-Hall, Inc., 718p.