Effects of Salinity on Survival, Oxygen Consumption, and Hematological Response of Greenling Hexagrammos otakii

The effects of salinity on survival, oxygen consumption, and hematological response of greenling Hexagrammos otakii, mean body weight 147.1±3.8 g, were investigated under salinity conditions of 33.4 (control), 33.1, 32.8, 32.2, 31.0, 28.7, 23.9, 14.5, and 3.8 psu, to evaluate physiological effects in relation to changes in salinity. The survival and hematological responses of fish were measured at each salinity after 96- and 24-h exposures. The oxygen consumption rate (OCR) of fish was measured in triplicate under conditions of stepwise salinity exposure (33.4→33.1→32.8→32.2→31.0→28.7→23.9→14.5→3.8 psu) with an interval of 24 h at each salinity, using a continuous flow-through respirometer. No fish mortality was observed in the range of 33.4 to 14.5 psu, but the survival rate was reduced to 53.3% at 3.8 psu after 96 h of exposure. The OCRs did not significantly differ in the range between 33.4 to 28.7 psu (P>0.05), but significantly increased at 23.9 and 14.5 psu, and then dramatically decreased at 3.8 psu compared to the control (P<0.05). Hematological variables, such as glucose, glutamic pyruvic transaminase (GPT), hematocrit, and Na⁺, were affected by reduced salinity. This result may be applicable for habitat and culture management of greenlings.

쥐노래미(Hexagrammos otakii)의 생존율, 산소 소비율과 혈액 성상에 미치는 염분의 영향