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http://dx.doi.org/10.5657/KFAS.2015.0064

Gill Na+/K+-ATPase Activity and Expression in Black Sea Bream Acanthopagrus schlegelii Exposed to a Hyposaline Environment  

Min, Byung Hwa (Aquaculture Research Institute, National Fisheries Research and Development Institute)
Park, Mi Seon (Aquaculture Research Institute, National Fisheries Research and Development Institute)
Myeong, Jeong-In (Aquaculture Research Institute, National Fisheries Research and Development Institute)
Seo, Jeong Soo (Aquaculture Research Institute, National Fisheries Research and Development Institute)
Park, Jung Jun (Aquaculture Research Institute, National Fisheries Research and Development Institute)
Noh, Gyeong Eon (Aquaculture Research Institute, National Fisheries Research and Development Institute)
Kang, Duk Young (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.48, no.1, 2015 , pp. 64-70 More about this Journal
Abstract
We investigated the branchial osmoregulatory response of black sea bream Acanthopagrus schlegelii to short-term (3-48 h) exposure to a hyposaline environment (5 psu). Gill $Na^+/K^+$-ATPase (NKA) activity was decreased after 3 h in fish transferred to 5 psu compared to salt water-acclimated (control) fish, but the level of activity returned to that observed in the control fish at 6 h after transfer. NKA activity increased significantly at 24 h after transfer, but it returned to the level observed in the control fish at 48 h after transfer. Immunohistochemical staining revealed that gill NKA was localized to chloride cells. The number of chloride cells tended to change in parallel with NKA activity. Substantial decreases in plasma $Na^+$, $Cl^-$, and osmolality were observed after 12 h of exposure to 5 psu; however, these parameters began to recover to the values detected in the controls at 24 h after transfer. In conclusion, our results suggest that black sea bream are able to adjust their osmoregulatory mechanisms to shift from hypo- to hyperosmoregulation within 6 h of exposure to a hypoosmotic environment.
Keywords
Acanthopagrus schlegelii; Osmoregulation; Gill; $Na^+/K^+$-ATPase activity; Immunohistochemical staining;
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