Browse > Article
http://dx.doi.org/10.5657/KFAS.2014.0882

Steroid Metabolism in the Blacktip Grouper Epinephelus fasciatus during Oocyte Vitellogenesis  

Kim, Seol Ki (Department of Marine Biology, Pukyong National University)
Baek, Hea Ja (Department of Marine Biology, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.47, no.6, 2014 , pp. 882-887 More about this Journal
Abstract
We studied oocyte steroidogenesis in blacktip grouper Epinephelus fasciatus ovarian follicles during vitellogenesis. Vitellogenic oocytes with average diameters of 0.45, 0.48 and 0.50 mm were incubated in vitro in the presence of $[^3H]17{\alpha}$-hydroxyprogesterone as a precursor. The steroid metabolites were analyzed using thin layer chromatography (TLC), high performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC/MS). The major metabolites in the vitellogenic oocytes were androstenedione ($A_4$), testosterone (T), estradiol-$17{\beta}$ ($E_2$), and estrone ($E_1$). The metabolites of androgen ($A_4$ and T) were higher in the 0.50-mm oocytes than in the 0.45- and 0.48-mm oocytes, while the estrogen metabolites (E2 and E1) were lower in the 0.50-mm oocytes. These results suggest that 0.50-mm oocytes are fully vitellogenic following initiation of the maturation process.
Keywords
Blacktip grouper; Epinephelus fasciatus Sex steroid hormones; Vitellogenesis;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Annalie VM, Roberts CM and Hawkins JP. 2000. The treatened status of groupers (Epinephelinae). Biodivers Conserve 9, 919-942.   DOI   ScienceOn
2 Campbell CM, Walsh JM and Idler DR. 1976. Steroids in the plasma of the winter flounder Pseudopleuronectes americanus Walbaum: a seasonal study and investigation of steroid involvement in oocyte maturation. Gen Comp Endocrinol 29, 14-20.   DOI
3 Fostier AB, Jalabert R, Billard R, Breton B and Zohar Y. 1983. The gonadal steroids. Fish Physiol 9A, 272-372.
4 Goos HJT, Facchinetti F, Henderson IW, Pierantoni R and Polzonetti-Magni AM. 1993. Pubertal development: big questions, small answers. Cell Comm Reprod, 11-20.
5 Hwang IJ, Kim SY and Baek HJ. 2007. Activity of sex steroid hormones on ovarian development in the greenling Hexagrammos otakii. J Kor Fish Soc 40, 153-159.   과학기술학회마을   DOI
6 Hwang IJ, Kim SK, Choi SJ, Lee CH, Lee YD, Kim HB and Baek HJ. 2012. Effects of steroid hormones on In vitro GVBD and oocyte steroidogenesis in blacktip grouper, Epinephelus fasciatus. Dev Reprod 16, 39-45.   과학기술학회마을
7 Kagawa H, Young C, Adachi, S and Nagahama Y. 1982. Estradiol-$17{\beta}$production in amago salmon (Oncorhynchus rhodurus) ovarian follicles: Role of the thecal layer and granulose cells. Gen Comp Endocrino 47, 440-448.   DOI   ScienceOn
8 Kawabe K and Kohno H. 2009. Morphological development of larval and juvenile blacktip grouper, Epinephelus fasciatus. Fish Sci 75, 1239-1251.   DOI   ScienceOn
9 Kang HC, Lee CH, Song YB, Baek HJ, Kim HB and Lee YD. 2012. KISS1, KISS2, GPR54 mRNA Expression of the Blacktip Grouper Epinephelus fasciatus. Dev Reprod 16, 121-128.   과학기술학회마을
10 Montero M, Le Belle N, King JA, Millar RP and Dufour S. 1995. Differential regulation of the two forms of gonadotropin releasing hormone (mGnRH and cGnRH-II) by sex steroids in the European female silver ell, Anguilla anguilla. Neuroendocrinology 61, 525-535.   DOI
11 Nagahama Y. 1994. 17-alpha, 20-beta-dihydrocy-4-pregnen-3-one, a maturation-inducing hormone in fish oocytes: mechanisms of synthesis and action. Steroids 62, 190-196.
12 Ohta K, Mine T, Yamaguchi A and Matsuyama M. 2001. Steroidogenic pathway to estradiol-$17{\beta}$ synthesis in the ovarian follicles of the protogynous wrasse, Pseudolabrus sieboldi. Zool Sci 18, 937-945.   DOI
13 Song YB, Baek HJ, Kim HB, Lee KJ, soyano K and Lee YD. 2005. Induced sex reversal of sevenband grouper, Epinephelus sepmtempasciatus by $17{\alpha}$-methyltestosterone. J Aquacult 18, 167-172.
14 Ohta K, Yamaguchi S, Yamaguchi A, Okuzawa K, Kagawa H and Matsuyama M. 2002. Biosynthsis of estradiol-$17{\beta}$ in the ovarin follicles of the red seabream Pagrus major during vitellogenesis. Fish Sci 68, 680-687.   DOI   ScienceOn
15 Park YJ, Kang HC, Lee CH, Song YB, Baek HJ, Kim HB, Soyano K and Lee YD. 2012. Isolation and identification of FSH$\beta$ and LH$\beta$ subunits in the blacktip grouper Epinephelus fasciatus. Kor J Fish Aquat Sci 45, 17-24.   과학기술학회마을   DOI
16 Patino R, Thomas P and Yoshizaki G. 2003. Ovarian follicle maturation and ovulation: and integrated perspective. Fish Physiol Biochem 28, 305-308.   DOI
17 Staub NL and De Beer. 1997. The role of androgens in female vertebrates. Gen Comp Endocrinol 108, 1-24.   DOI
18 Tyler CR, Sumpter JP and Bromage NR. 1988. Selectivity of protein sequestration by vitellogenic oocytes of rainbow trout, Salmo gairdneri. J Exp Zool 248, 199-206.   DOI
19 Yoshizaki G, Shusa M, Takeuchi T and Patino R. 2001. The induction of oocyte maturational competence by gonadotropin in Nibe, Nibea mitsukurii (Teleostei, Sciaenidae) requires activation of the protein kinase A pathway, and RNA and protein synthesis. Fish Physiol Biochem 25, 201-208.   DOI