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http://dx.doi.org/10.5657/KFAS.2014.0874

Chronic Effects of Copper on Antioxidant Enzymes and Acetylcholinesterase Activities in Rock bream Oplegnathus fasciatus  

Min, EunYoung (Institute of Fisheries science, PuKyong National University)
Kang, Ju-Chan (Department of Aquatic life medicine, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.47, no.6, 2014 , pp. 874-881 More about this Journal
Abstract
A laboratory experiment was conducted to determine chronic effects of waterborne copper exposure on rock bream Oplegnathus fasciatus using a panel of enzymes. The activities of the following biochemical biomarkers were determined at different concentrations of $CuSO_4$ for 10 and 20 days: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) in plasma; antioxidant enzymes including glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in liver and gills; and acethylcholinesterase (AChE) in brain and muscle. After exposure to two $CuSO_4$ concentrations (200 and $400{\mu}g/L$), the activities of plasma ALT in the fish showed a tendency to increase with AST and LDH, depending on $CuSO_4$ concentration. Additionally, GSH levels and SOD activities significantly increased, depending on $CuSO_4$ concentrations in liver and gills. This involved the inactivation of reactive molecules formed during oxidative stress, which could provide protection against oxidative damage induced by $CuSO_4$. However, GPx and AChE activities significantly decreased with $CuSO_4$ in liver and gills. In conclusion, these enzymes may represent convenient biomarkers for monitoring heavy metal pollution in coastal areas. Such chronic exposure studies are necessary for improving our understanding of complementary or deleterious effects of pollutants, and for developing metal toxicity biomarkers.
Keywords
Copper; GSH; Anti-oxidant enzymes; AChE; Oplegnathus fasciatus;
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