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http://dx.doi.org/10.5657/KFAS.2014.0853

Sperm Cryopreservation of Tetraploid Pacific Oysters Crassostrea gigas  

Park, Mi Seon (Aquaculture Management Division, National Fisheries Research and Development Institute)
Min, Byung Hwa (Aquaculture Management Division, National Fisheries Research and Development Institute)
Lim, Hyun-Jeong (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Hur, Young Baek (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Do, Yong Hyun (Aquaculture Management Division, National Fisheries Research and Development Institute)
Myeong, Jeong-In (Aquaculture Management Division, National Fisheries Research and Development Institute)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.47, no.6, 2014 , pp. 853-859 More about this Journal
Abstract
The goal of this study was to evaluate the effects of cryoprotectants [dimethyl sulfoxide (DMSO), methanol, polyethylene glycol, and propylene glycol], cryoprotectant concentrations (10% and 20%), equilibration time (3, 10, and 30 min), cooling rate ($3^{\circ}$, $5^{\circ}$, $7^{\circ}$, and $10^{\circ}/min$), and straw size (0.25 and 0.5 mL) for sperm cryopreservation of tetraploid Pacific oysters. There was a significant difference among the four cryoprotectants, with 10% DMSO yielding the highest post-thaw survival and activity index of sperm. A significant relationship was observed between the cryoprotectant and its concentration. The sperm with equilibration times of 30 min yielded higher post-thaw survival and activity indices than those with 3 and 10 min equilibration times. The sperm cooled at a rate of $5^{\circ}/min$ yielded the highest post-thaw survival and activity index, and the results were significantly different from those observed for cooling at $7^{\circ}$ and $10^{\circ}/min$. Post-thaw survival and activity indices of sperm using a 0.25-mL straw were significantly higher than those using a 0.5-mL straw.
Keywords
Tetraploid; Pacific oyster; Crassostrea gigas; Sperm; Cryopreservation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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1 Adams SL, Smith JF, Roberts RD, Janke AR, Kaspar HF, Tervit HR, Pugh PA, Webb SC and King NG. 2004. Cryopreservation of sperm of the Pacific oyster (Crassostrea gigas):development of a practical method for commercial spat production. Aquaculture 242, 271-282.   DOI   ScienceOn
2 Babiak I, Fraser L, Dobosz S, Goryczko K, Kuzminski H and Strzezek J. 1999. Computercontrolled freezing of rainbow trout Oncorhnchus mykiss (Walbaum) spermatozoa for routine programmes. Aquacult Res 30, 707-710.   DOI
3 Billard R and Zhang T. 2001. Techniques of genetic resource banking in fish. In: Cryobanking the Genetic Resource:Wildlife Conservation for the Future. Walson PF and Holt WV, eds. Taylor & Francis, New York, USA, 143-170.
4 Cabrita E, Robles V, Alvarez R and Herraez MP. 2001. Cryopreservation of rainbow trout sperm in large volume straws: application to large scale fertilization. Aquaculture 201, 301-314.   DOI   ScienceOn
5 Christensen JM and Tiersch TR. 1996. Refrigerated storage of channel catfish sperm. J World Aquacult Soc 3, 340-346.
6 Christensen JM and Tiersch TR. 1997. Cryopreservation of channel catfish spermatozoa: effect of cryopreservation, straw size, and formulation of extender. Theriogenology 47, 639-645.   DOI
7 Dong Q, Eudeline B, Huang C, Allen Jr SK and Tiersch TR. 2005a. Commercial-scale sperm cryopreservation of diploid and tetraploid Pacific oyster, Crassostrea gigas. Cryobiology 50, 1-16.   DOI
8 Dong Q, Huang C, Eudeline B, Allen Jr SK and Tiersch TR. 2006. Systematic factor optimization for sperm cryopreservation of tetraploid Pacific oyster, Crassostrea gigas. Theriogenology 66, 387-403.   DOI
9 Dong Q, Huang C, Eudeline B and Tiersch TR. 2005b. Systematic factor optimization for cryopreservation of shipped sperm samples of diploid Pacific oyster, Crassostrea gigas (Thunberg). Cryobiology 51, 176-197.   DOI   ScienceOn
10 Dong Q, Huang C and Tiersch TR. 2005c. Spermatozoal ultrastructure of diploid and tetraploid Pacific oyster. Aquaculture 249, 487-496.   DOI
11 Eudeline B, Allen Jr SK and Guo X. 2000a. Optimization of tetraploid induction in Pacific oysters, Crassostrea gigas, using first polar body as a natural indicator. Aquaculture 187, 73-84.   DOI
12 Eudeline B, Allen Jr SK and Guo X. 2000b. Delayed meiosis and polar body release in eggs of triploid Pacific oysters, Crassostrea gigas, in relation to tetraploid production. J Exp Mar Biol Ecol 248, 151-161.   DOI
13 Guo X and Allen Jr SK. 1994. Viable tetraploids in the Pacific oyster (Crassostrea gigas Thunberg) produced by inhibition of polar body I in eggs from triploids. Mol Mar Biol and Biotechnol 3, 42-50.
14 Guo X, Debrosse G and Allen Jr SK. 1996. All-triploid Pacific oysters (Crassostrea gigas Thunberg) produced by mating tetraploids and diploids. Aquaculture 142, 149-161.   DOI
15 Huang C, Dong Q, Walter RB and Tiersch TR. 2004. Sperm cryopreservation of green swordtail Xiphophorus helleri, a fish with internal fertilization. Cryobiology 48, 295-308.   DOI
16 Hughes JB. 1973. An examination of eggs challenged with cryopreserved spermatozoa of the American oyster, Crassostrea virginica. Cryobiology 10, 342-344.   DOI
17 Kuwano K and Saga N. 2000. Cryopreservation of marine algae:Cryopreservation of marine algae: Applications in biotechnology. In: Recent advances in marine biotechnology. Figerman M and Nagabhusshanam R, eds. Volume 4:Aquaculture. Part A, Seaweeds and invertebrates. Science Pubulishers Inc., New Hampshire, 23-40.
18 Hwang SW and Chen HP. 1973. Fertility of male oyster gametes after freeze-thawing. Chinese-American Joint Commission on Rural Reconstruction Fisheries Series 15, 1-5.
19 Jamieson BGM. 1991. Fish evolution and systematics: Evidence from spermatozoa. With a survey of lophophorate, echinodern and photochordate sperm and an account of gamete cryopreservation. Cambridge University Press, Cambridge, xiv 319.
20 Kho KH. 2007. Effects of cryoprotectants and diluents on cryopreservation of the red seabream, Pagrus major sperm. Korean J Ichthyol 19, 173-177.   과학기술학회마을
21 McFadzen IRB. 1995. Cryopreservation of sperm of the Pacific oyster Crassostrea gigas. In: Cryopreservation and Freeze-Drying Protocols. Day JG and McLellan MR, eds. Humana Press, Totowa, New Jersey, U.S.A., 145-149.
22 Meryman HT. 1971. Cryoprotective agents. Cryobiology 8, 173-183.   DOI
23 Park MS, Lim HJ and Lee TS. 1999. Biological and chemical characterization of triploid Pacific oyster (Crassostrea gigas) in the waters of Tongyoung, Korea. Bull Fish Res Dev Agency 55, 31-40.
24 Park MS, Min BH, Park JJ, Lim HJ, Myeong JI and Jeong MH. 2013. Cryopreservation of Pacific oyster, Crassostrea gigas sperm. Korean J Malacol 29, 251-258.   과학기술학회마을   DOI
25 Richardson GF, Wilson CE, Crim LW and Yao Z. 1999. Cryopreservation of yellowtail flounder (Pleuronectes ferrugineus) semen in large straws. Aquaculture 174, 89-94.   DOI
26 Pickett BW and Berndton WE. 1974. Preservation of bovine spermatozoa by freezing in straws: A review. J Dairy Sci 57, 1287-1301.   DOI
27 Piironen J. 1993. Cryopreservation of sperm from brown trout (Salmon trutta m. lacustris L.) and Arctic charr (Salvelinus alpinus L.). Aquaculture 116, 275-285.   DOI
28 Richardson GF, Miller TL and McNiven MA. 2000. Cryopreservation of Arctic charr, Salvelinus alpinus (L.), semen in various extenders and in three sizes of straw. Aquacult Res 31, 307-315.   DOI
29 Sansone G, Fabbrocini A, Ieropoli S, Langellotti A, Occidente M and Matassino D. 2002. Effects of extender composition, cooling rate, and freezing on the motility of sea bass (Dicentrarchus labrax, L.) spermatozoa after thawing. Cryobiology 44, 229-239.   DOI
30 Stanley JG, Hidu H and Allen Jr SK. 1984. Growth of American oysters increased by polyploidy induced by blocking meiosis I but not meiosis II. Aquaculture 37, 147-155.   DOI
31 Strüssmann CA, Renard P, Ling H and Takashima F. 1994. Motility of pejerrey Odontesthes bonariensis spermatozoa. Fish Sci 60, 9-13.   DOI
32 Tiersch TR. 2000. Introduction. In: Cryopreservation in Aquatic Species. Tiersch TR and Mazik PM, eds. World Aquaculture Society, Baton Rouge, Louisiana. 19-26.
33 Wang Z, Guo X, Allen Jr SK and Wang R. 1999. Aneuploid Pacific oyster (Crassostrea gigas Thunberg) as incidentals from triploid production. Aquaculture 173, 347-357.   DOI