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http://dx.doi.org/10.5657/KFAS.2014.0516

Biochemical Characterization of α-Galactosidase-Producing Thermophilic Bacillus coagulans KM-1  

Nam, Ki Ho (Food and safety Research Division, National Fisheries Research and Development Institute)
Jang, Mi Soon (Food and safety Research Division, National Fisheries Research and Development Institute)
Park, Hee Yeon (Food and safety Research Division, National Fisheries Research and Development Institute)
Koneva, Elena (TINRO Centre, Pacific Scientific Research Fisheries Centre, Shevchenko Alley)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.47, no.5, 2014 , pp. 516-521 More about this Journal
Abstract
A bacterium producing ${\alpha}$-galactosidase (${\alpha}$-$\small{D}$-galactoside galactohydrolase, EC 3.2.1.22) was isolated. The isolate, KM-1 was identified as Bacillus coagulans based on its 16S rRNA sequence, morphology, and biochemical properties. ${\alpha}$-Galactosidase activity was detected the culture supernatant of B. coagulans KM-1. The bacterium showed the maximum activity for hydrolyzing para-nitrophenyl-${\alpha}$-$\small{D}$-galactopyranoside (pNP-${\alpha}Gal$) at pH 6.0 and $50^{\circ}C$. It hydrolyzed oligomeric substrates such as melibiose, raffinose, and stachyose liberating a galactose residue, indicating that the B. coagulans KM-1 ${\alpha}$-galactosidase hydrolyzed ${\alpha}$-1,6 linkage. The results suggest that the decreased stachyose and raffinose contents in fermented soybean meal are due to the ${\alpha}$-galactosidase activity.
Keywords
Bacillus coagulans KM-1; ${\alpha}$-galactosidase; Stachyose; Raffinose; Fermented soybean meal;
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