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http://dx.doi.org/10.5657/KFAS.2014.0144

Effect of Culture Conditions on Characteristics of Growth and Production of Docosahexaenoic acid (DHA) by Schizochytrium mangrovei  

Jeong, U-Cheol (Department of Marine Biology and Aquaculture/Institute of Marine Industry, Gyeongsang National University)
Choi, Byeong-Dae (Department of Seafood Science and Technology, Gyeongsang National University)
Kang, Seok-Joong (Department of Marine Biology and Aquaculture/Institute of Marine Industry, Gyeongsang National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.47, no.2, 2014 , pp. 144-153 More about this Journal
Abstract
Both docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3) have attracted increasing attention since the first epidemiological report on the importance of n-3 essential fatty acids. Lipids in microbial cells play various biological roles and, consequently, much research has been carried out on their role in cell physiology. The lipid composition of microorganisms can exhibit considerable variations depending on environment. The effects of culture conditions, temperature (15, 20, 24, 28, 32 and $36^{\circ}C$), salinity (10, 20, 30, 40 and 50 psu), pH (pH5, 6, 7, 8 and 9), rotation speeds (50, 100, 150 and 200 rpm), carbon sources, nitrogen sources and C/N ratio on the production of docosahexaenoic acid, fatty-acid profiles, and acids secreted to the broth culture by the oleaginous microorganism, Schizochytrium mangrovei (KCTC 11117BP), were studied. Temperature (initially $28^{\circ}C$), salinity (20 psu), pH (pH7), rotation speeds (100 rpm), organism fatty acids, and secreted acids in the broth were varied during cultivation of S. mangrovei. At pH 7.0, S. mangrovei was able to accumulate lipids up to 40% of its biomass, with 13% (w/w) DHA content. The monosaccharides glucose and fructose, and yeast extract were suitable carbon and nitrogen sources, respectively. The primary omega-3 polyunsaturated fatty acid produced was docosahexaenoic acid.
Keywords
Schizochytrium mangrovei; Docosahexaenoic acid; Omega-3 fatty acid; Marine Microorganism;
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Times Cited By KSCI : 2  (Citation Analysis)
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